Sparc/osteonectin, cwcv, and kazal-like domains proteoglycan 1 (SPOCK1) has been shown to promote various tumors, but its role in colon cancer (CRC) has not been clearly illuminated. The aim of this study was to investigate the effects of SPOCK1 interference on the proliferation, migration and EMT of CRC cells. First, we analyzed the expression of SPOCK1 in various CRC datasets. Then, we investigated the correlation between SPOCK1 and prognosis in CRC patients. We overexpressed SPOCK1 and knocked down SPOCK1 expression in HCT-116 and SW480 cells, respectively. Then, cell proliferation was assayed with a CCK-8 assay, and cell migration was evaluated with a Transwell migration assay. NF-κB and EMT-related proteins were studied by western blotting. The results indicated that the mRNA levels of SPOCK1 were relatively high in CRC tissues and that high expression of SPOCK1 was negatively correlated with patient prognosis. With SPOCK1 overexpression in HCT-116 cells, cell proliferation and migration were increased, while SPOCK1 knockdown had the opposite effects. With SPOCK1 overexpression in HCT-116 cells, the expression levels of NF-κB and EMT-related proteins were elevated, while SPOCK1 knockdown produced the opposite results. In conclusion, our study demonstrates that SPOCK1 may activate the NF-κB/Snail signaling cascade to promote the proliferation and migration of CRC cells. SPOCK1 may serve as a new prognostic indicator and potential therapeutic target in CRC.

中文翻译：

---

SPOCK1通过调节NF-κB通路和诱导EMT促进结肠癌细胞的增殖和迁移。

Sparc/骨连接蛋白、cwcv 和 kazal 样结构域蛋白多糖 1 (SPOCK1) 已被证明可促进各种肿瘤，但其在结肠癌 (CRC) 中的作用尚未明确阐明。本研究旨在探讨SPOCK1干扰对CRC细胞增殖、迁移和EMT的影响。首先，我们分析了 SPOCK1 在各种 CRC 数据集中的表达。然后，我们研究了 SPOCK1 与 CRC 患者预后之间的相关性。我们分别在 HCT-116 和 SW480 细胞中过表达 SPOCK1 并敲低了 SPOCK1 的表达。然后，用CCK-8测定法测定细胞增殖，并用Transwell迁移测定法评估细胞迁移。通过蛋白质印迹研究 NF-κB 和 EMT 相关蛋白。结果表明，结直肠癌组织中SPOCK1的mRNA水平较高，SPOCK1的高表达与患者预后呈负相关。SPOCK1 在 HCT-116 细胞中过表达，细胞增殖和迁移增加，而 SPOCK1 敲低具有相反的效果。SPOCK1 在 HCT-116 细胞中过表达时，NF-κB 和 EMT 相关蛋白的表达水平升高，而 SPOCK1 敲低产生相反的结果。总之，我们的研究表明，SPOCK1 可能激活 NF-κB/Snail 信号级联以促进 CRC 细胞的增殖和迁移。SPOCK1 可作为 CRC 的新预后指标和潜在治疗靶点。细胞增殖和迁移增加，而 SPOCK1 敲低具有相反的效果。SPOCK1 在 HCT-116 细胞中过表达时，NF-κB 和 EMT 相关蛋白的表达水平升高，而 SPOCK1 敲低产生相反的结果。总之，我们的研究表明，SPOCK1 可能激活 NF-κB/Snail 信号级联以促进 CRC 细胞的增殖和迁移。SPOCK1 可作为 CRC 的新预后指标和潜在治疗靶点。细胞增殖和迁移增加，而 SPOCK1 敲低具有相反的效果。SPOCK1 在 HCT-116 细胞中过表达时，NF-κB 和 EMT 相关蛋白的表达水平升高，而 SPOCK1 敲低产生相反的结果。总之，我们的研究表明，SPOCK1 可能激活 NF-κB/Snail 信号级联以促进 CRC 细胞的增殖和迁移。SPOCK1 可作为 CRC 的新预后指标和潜在治疗靶点。我们的研究表明，SPOCK1 可能激活 NF-κB/Snail 信号级联以促进 CRC 细胞的增殖和迁移。SPOCK1 可作为 CRC 的新预后指标和潜在治疗靶点。我们的研究表明，SPOCK1 可能激活 NF-κB/Snail 信号级联以促进 CRC 细胞的增殖和迁移。SPOCK1 可作为 CRC 的新预后指标和潜在治疗靶点。