An eDNA assay for the detection of Pacific herring (Clupea pallasii) in water samples was developed using quantitative PCR (qPCR) technology. Species-specific primers and a minor groove binding (MGB) probe were designed based on the mitochondrial cytochrome b gene sequence. Upon conducting a cross-species test, the target species DNA was not only detected at low concentrations but also with high specificity. The assay was validated using field-collected water samples from aquatic environments known to be inhabited by Pacific herring and tested positive in samples from Pacific herring-inhabited sites. Our in silico, in vitro, and in situ results highlight the sensitivity of our proposed eDNA-based procedure for the detection of C. pallasii DNA, and could provide a non-invasive and non-destructive approach to detect this species and characterize its distribution in the Jinhaeman Bay, Korea.

使用定量PCR（qPCR）技术开发了一种用于检测水样中太平洋鲱鱼（Clupea pallasii）的eDNA分析法。基于线粒体细胞色素b基因序列设计了物种特异性引物和小沟结合（MGB）探针。通过进行跨物种测试，不仅可以以低浓度检测到目标物种的DNA，而且还可以以高特异性检测出目标物种的DNA。使用从已知被太平洋鲱鱼栖息的水生环境中现场采集的水样本验证了该测定，并在太平洋鲱鱼栖息地的样本中检测出阳性。我们的计算机模拟，体外和原位结果突出了我们提出的基于eDNA的程序对帕氏疟原虫的检测的敏感性 DNA，并可以提供一种非侵入性和非破坏性的方法来检测该物种并表征其在韩国金海曼湾的分布。