Retinoblastoma is known as childhood rare malignancy of the retina. Ciliary neurotrophic factor (CNTF) was previously found to reduce degeneration and promote retina survival. This work investigated the effects of CNTF supplementation on in-vitro model cells including retinoblastoma (Y79) and adipose-derived mesenchymal stem cells (AMSCs) viability, proliferation, gene expression and cell cycle. A drop of viability was detected in Y79 treated with CNTF in a dose-dependent manner (P < .05). However, the proliferation of AMSCs was increased at lower concentrations of CNTF (5 ng/mL), but declined in higher doses (50 and 100 ng/mL). The BrdU assay confirmed the MTT assay results. Cell cycle was arrested in both Y79 and AMSCs in the G0/G1 phase by CNTF treatment. A considerable down-regulation of Bcl2, CycD1 and N-Myc genes expression (P < .05) inversely, P15 and P21 genes up-regulation in treated Y79 cells was observed. Besides, stemness genes' transcription was reduced in AMSCs (P < .05), and levels of neuronal-specific markers such as neuron-specific enolase (NSE) and neuronal nuclei (NeuN) were increased (P < .05). The findings of this study suggest a promising potential of CNTF in terms of arresting Y79 retinoblastoma cells, and differentiation-inducing to AMSCs, which could be valuable for managing future innovative treatments targeting retinoblastoma.

中文翻译：

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外源性睫状神经营养因子对体外模型细胞细胞周期和神经分化标志物的影响：对未来治疗方法的新见解

视网膜母细胞瘤被称为儿童期罕见的视网膜恶性肿瘤。先前发现睫状神经营养因子 (CNTF) 可减少变性并促进视网膜存活。这项工作研究了 CNTF 补充剂对体外模型细胞的影响，包括视网膜母细胞瘤 (Y79) 和脂肪来源的间充质干细胞 (AMSC) 的活力、增殖、基因表达和细胞周期。在以剂量依赖的方式在用 CNTF 处理的 Y79 中检测到活力下降（P < .05）。然而，AMSCs 的增殖在较低浓度的 CNTF (5 ng/mL) 下增加，但在较高剂量 (50 和 100 ng/mL) 下下降。BrdU 测定证实了 MTT 测定结果。通过CNTF处理，在G0/G1期的Y79和AMSC中的细胞周期被阻滞。 在处理过的 Y79 细胞中观察到Bcl2、CycD1 和 N-Myc 基因表达显着下调（P < .05），相反，P15 和 P21 基因上调。此外，AMSCs中干性基因的转录减少（P  < .05），神经元特异性标志物如神经元特异性烯醇化酶（NSE）和神经元核（NeuN）的水平增加（P < .05）。这项研究的结果表明 CNTF 在阻止 Y79 视网膜母细胞瘤细胞和诱导 AMSCs 分化方面具有广阔的潜力，这对于管理未来针对视网膜母细胞瘤的创新治疗可能很有价值。