Abstract

Phellinus strains were collected from different areas in Korea. Of them, the fast mycelial growing strains were artificially cultivated on the oak logs to produce fruiting body. The varieties, Phellinus linteus ASI26099 (Korea Sanghwang) and P. baumii PBJS (Jangsoo Sanghwang) were grown under the same conditions as controls. Their cultivating characteristics including mycelial colonization, pinhead formation, and fruiting body formation rate were investigated on the logs. Basidiocarps of Phellinus strains HN00K9, HN6036, and ASI26099 were concentrically zonate and shallowly sulcate, and dark chestnut showing typical characteristics of Tropicoporus linteus (synonyum: P. linteus, Inonotus linteus, polyporus linteus), which is distinguishably different to PBJS. HN00K9 showed the highest yield of fruiting body among the mushroom strains. The β-glucan content in fruiting bodies of HN00K9 was 20% higher than those of other strains. Bioactive effects of polysaccharide samples from fruiting bodies of Phellinus strains, HN00K9, HN6036, ASI26099, and PBJS were assessed on cell viability and cytokine (IL-6 and TNF-α) inhibition and finally on anticancer to different human cancer cells.

摘要

桑黄菌株是从韩国不同地区采集的。其中，菌丝生长快的菌株是在橡木原木上人工培养产生子实体的。桑黄品种ASI26099（韩国 Sanghwang）和鲍米白杨PBJS（Jangsoo Sanghwang）在与对照相同的条件下生长。在原木上研究了它们的培养特性，包括菌丝定植、针头形成和子实体形成率。桑黄菌株HN00K9、HN6036和ASI26099的担子果呈同心环带状，浅具沟，深栗色，表现出热带多孔菌（同义：P. linteus、桦褐孔菌、多孔菌）的典型特征，与PBJS有明显区别。HN00K9 在蘑菇菌株中子实体产量最高。HN00K9子实体中β-葡聚糖含量比其他菌株高20%。评估桑黄菌株 HN00K9、HN6036、ASI26099 和 PBJS 子实体多糖样品对细胞活力和细胞因子（IL-6 和 TNF-α）抑制的生物活性作用，最后评估对不同人类癌细胞的抗癌作用。