Sulfur (S) is an essential macronutrient required by plants. Plants absorb and transport S through sulfate transporters (SULTRs). In this study, we cloned 8 SULTR genes (CsSULTR1;1/1;2/2;1/3;1/3;2/3;3/3;5/4;1) from tea plant (Camellia sinensis), all of which contain a typical sulfate transporter and antisigma factor antagonist (STAS) conserved domain. Phylogenetic tree analysis further divided the CsSULTRs into four main groups. Many cis-acting elements related to hormones and environmental stresses were found within the promoter sequence of CsSULTRs. Subcellular localization results showed that CsSULTR4;1 localized in the vacuolar membrane and that other CsSULTRs localized to the cellular membrane. The tissue-specific expression of the 8 CsSULTR genes showed different expression patterns during the active growing period and dormancy period. In particular, the expression of CsSULTR1;1 was highest in the roots, but that of CsSULTR1;2 was lowest in the dormancy period. The expression of CsSULTR1;1/1;2/2;1/3;2 was stimulated under different concentrations of selenium (Se) and S; moreover, CsSULTR1;2/2;1/3;3/3;5 was upregulated in response to different valences of Se.

硫 (S) 是植物所需的必需常量营养素。植物通过硫酸盐转运蛋白 (SULTRs) 吸收和转运硫。本研究从茶树（Camellia sinensis ）中克隆了8个SULTR基因（ CsSULTR1;1/1;2/2;1/3;1/3;2/3;3/3;5/4;1），所有这些都包含一个典型的硫酸盐转运蛋白和抗西格玛因子拮抗剂 (STAS) 保守结构域。系统发育树分析进一步将 CsSULTR 分为四个主要组。在CsSULTRs的启动子序列中发现了许多与激素和环境压力相关的顺式作用元件。亚细胞定位结果表明，CsSULTR4;1 定位于液泡膜，而其他 CsSULTRs 定位于细胞膜。8 CsSULTR的组织特异性表达基因在活跃生长期和休眠期表现出不同的表达模式。特别是CsSULTR1;1在根部的表达量最高，而CsSULTR1;2在休眠期的表达量最低。CsSULTR1;1/1;2/2;1/3;2的表达在不同浓度的硒（Se）和S下受到刺激；此外，CsSULTR1;2/2;1/3;3/3;5响应不同价的硒而上调。