Sweet corn has gained worldwide popularity. Traditional sweet corn possesses low concentration of essential nutrients such as lysine (0.15–0.25%), tryptophan (0.03–0.04%) and provitamin-A (proA 3–4 ppm), and deficiency leads to serious health problems in humans. Here, stacking of shrunken2 (sh2), opaque2 (o2), lycopene epsilon cyclase (lcyE) and β‐carotene hydroxylase (crtRB1) genes were undertaken in the parents of four hybrids viz., APQH1, APHQ4, APHQ5 and APHQ7 using marker-assisted backcross breeding (MABB). Gene-linked markers (umc2276 and umc1320) for sh2, while gene-based markers for o2 (umc1066 and phi057), lcyE (5′TE-InDel) and crtRB1 (3′TE-InDel), were used for genotyping in BC₁F₁, BC₂F₁ and BC₂F₂. Selected backcross progenies showed high recovery of recurrent parent genome (92.4–97.7%). The reconstituted sweet corn hybrids possessed significantly high lysine (0.390%), tryptophan (0.082%) and proA (21.14 ppm), coupled with high kernel sweetness (brix 18.96%). The improved sweet corn hybrids had high cob yield (12.22–15.33 t/ha) across three environments. These newly developed biofortified sweet corn hybrids possess great significance in providing balanced nutrition. This is the first report of combining sh2, o2, lcyE and crtRB1 genes for enrichment of sweet corn hybrids with multiple essential nutrients.

甜玉米已在世界范围内广受欢迎。传统甜玉米含有低浓度的必需营养素，如赖氨酸 (0.15–0.25%)、色氨酸 (0.03–0.04%) 和维生素原 A (proA 3–4 ppm)，缺乏会导致严重的人类健康问题。在这里，堆叠的shrunken2（SH2），opaque2（O2），番茄红素的ε-环化酶 （lcyE）和β胡萝卜素羟化酶 （crtRB1）基因在四个杂交父母承担即，APQH1，APHQ4，APHQ5和APHQ7使用标记-辅助回交育种 (MABB)。基因连锁标记（umc2276和umc1320) 用于sh2，而o2（umc1066和phi057）、lcyE（5'TE-InDel）和crtRB1（3'TE-InDel）的基因标记用于BC ₁ F ₁、BC ₂ F ₁和BC ₂ F ₂. 选定的回交后代显示出重复亲本基因组的高回收率 (92.4–97.7%)。重组的甜玉米杂交种具有显着高的赖氨酸 (0.390%)、色氨酸 (0.082%) 和 proA (21.14 ppm)，以及高核甜度 (白利糖度 18.96%)。改良的甜玉米杂交种在三种环境中都具有高玉米芯产量（12.22-15.33 吨/公顷）。这些新开发的生物强化甜玉米杂交种在提供均衡营养方面具有重要意义。这是结合sh2、o2、lcyE和crtRB1基因用于富集具有多种必需营养素的甜玉米杂交种的第一份报告。