This report was aimed to study the validity of vitamins A and E on the spermatogenesis disorders caused by titanium dioxide nanoparticles (TiO2 NPs). Thirty-six male Wistar rats were randomly divided into six groups. Two groups as controls received bi-distilled water and olive oil for three weeks, respectively. The third group treated with 300 mg/kg TiO2 NPs for two weeks. The other groups received vitamin E (100 IU/kg), vitamin A (100 IU/kg), and the combination of these vitamins for 3 weeks, respectively. At the end of the study, sperm parameters, sperm chromatin integrity, and testicular histopathology changes were assessed. Sperm count, motility, and viability significantly decreased in TiO2 NPs-intoxicated rats (p < 0.05). Sperm chromatin staining also showed that spermatozoa with the impaired chromatin were upper in the nanoparticle receiving group matched to the controls (p < 0.05). Histopathological lesions like inflammation were found in the group treated by TiO2 NPs compared to the controls (p < 0.05). Accordingly, vitamins E and A could significantly prevent these changes (p < 0.05). The combination of the two vitamins showed a better performance than the separate administration of each one. The results of this study illustrated that vitamins E and A have a potent protective effect against testicular toxicity.

本报告旨在研究维生素A和E在二氧化钛纳米颗粒（TiO2 NPs）引起的精子生成疾病中的有效性。将三十六只雄性Wistar大鼠随机分为六组。两组作为对照组分别接受双蒸馏水和橄榄油三个星期。第三组用300 mg / kg TiO2 NP进行了两周的治疗。其他组分别接受维生素E（100 IU / kg），维生素A（100 IU / kg）和这些维生素的组合治疗3周。在研究结束时，评估了精子参数，精子染色质完整性和睾丸组织病理学变化。TiO2 NPs中毒大鼠的精子数量，运动能力和生存力显着降低（p <0.05）。精子染色质染色还显示，染色质受损的精子在与对照组相匹配的纳米颗粒接受组中较高（p  <0.05）。与对照组相比，在用TiO2 NPs治疗的组中发现了诸如炎症的组织病理学病变（p  <0.05）。因此，维生素E和A可以显着阻止这些变化（p  <0.05）。两种维生素的组合显示出比单独使用每种维生素更好的性能。这项研究的结果表明，维生素E和A对睾丸毒性具有有效的保护作用。