Anti-Müllerian hormone (AMH) is known as a reliable marker of ovarian reserve (OR). The determination of AMH is of great importance and most existed AMH detection methods are heterogeneous immunoassay. In this study, a novel homogeneous sandwich immunoassay-light-initiated chemiluminescence assay (LICA) for detecting AMH serum level was developed. This AMH-LICA was performed by incubating serum samples with AMH mouse monoclonal antibody coated with chemibeads, streptavidin-coated sensibeads, and biotinylated AMH mouse monoclonal antibody. Sensitivity, precision, accuracy and cross-reactivity of this assay were evaluated. Besides, a regression analysis showed a high correlation between AMH-LICA and Roche Elecsys® AMH assay (y = 0.9851x + 0.07147, R² = 0.9569). As a homogeneous immunoassay, this AMH-LICA could accurately and rapidly determine the serum level of AMH with high-throughput. Thus, this new developed assay may be a new useful analytical tool for the determination of AMH.

抗苗勒管激素 (AMH) 被称为卵巢储备 (OR) 的可靠标志物。AMH的测定非常重要，现有的大多数AMH检测方法是异质免疫分析。在这项研究中，开发了一种用于检测 AMH 血清水平的新型均质夹心免疫测定 - 光引发化学发光测定 (LICA)。这种 AMH-LICA 是通过将血清样品与涂有化学珠的 AMH 小鼠单克隆抗体、链霉亲和素包被的 sensibeads 和生物素化 AMH 小鼠单克隆抗体一起孵育来进行的。评估了该测定的灵敏度、精确度、准确度和交叉反应性。此外，回归分析显示 AMH-LICA 与 Roche Elecsys® AMH 检测之间存在高度相关性 ( y  = 0.9851 x  + 0.07147, R ² = 0.9569）。作为均相免疫测定，该AMH-LICA可以高通量准确、快速地测定血清AMH水平。因此，这种新开发的测定法可能是测定 AMH 的一种新的有用分析工具。