Abstract

Date palm pollen (DPP) has been widely tested in vitro in preservation of spermatozoa of different farm animals except in rabbits. The objective of this study was to evaluate the effect of various concentrations (20, 40, 80 mg/mL) of DPP aqueous extract on epididymal and ejaculated rabbit spermatozoa during in vitro incubation at 37 °C during 120 min. NaCl Pollen Extender (NPE) and TRIS Base Extender (TPE) were respectively used in epididymal and ejaculated sperm preservation. Sperm motility parameters were objectively analysed by a Computer Aided Sperm Analyser (CASA). The results revealed that in epididymal sperm at 0 minute, sperm motility showed significant higher values (p ≤ 0.05) for all doses of DPP in comparison to the control group. After 30, 60 and 120 min, the motility rate was higher (p > .05) in all the experimental groups compared to the control. Concerning velocity traits (VCL and VAP), and trajectories parameters (LIN, ALH, BCF), the doses of 40 mg/mL and 80 mg/mL showed the highest values than Control. In ejaculated samples, the motility rate at 0 minute improved only with the dose of 80 mg/mL and DPP showed protective effect at all doses (20, 40, 80 mg/mL) up to 60 min of incubation. DPP enhanced spermatozoa velocity (VCL and VAP) and the trajectory of sperm (LIN, ALH, BCF) even from 20 mg/mL at any points of storage. In conclusion, aqueous extract of date palm pollen is a suitable supplement to the extender for rabbit sperm by protecting and improving sperm motility parameters.

• HIGHLIGHTS

• The addition of DPP extract improves the quality of epididymal and ejaculated rabbit sperm.

• The extender supplemented with 80 mg/mL of DPP in NPE has a beneficial effect on kinetic traits of epididymal sperm. Nevertheless, in TPE extender, 20 mg/mL of DPP was enough to improve sperm motility traits in ejaculated rabbit semen.

摘要

枣棕榈花粉（DPP）已在体外进行了广泛测试，可以保存除兔子以外的其他各种农场动物的精子。这项研究的目的是评估在37°C下120分钟的体外温育过程中，各种浓度（20、40、80 mg / mL）的DPP水提取物对附睾和射精的兔精子的影响。NaCl花粉扩展剂（NPE）和TRIS基础扩展剂（TPE）分别用于附睾和射精精子的保存。精子运动参数通过计算机辅助精子分析仪（CASA）进行客观分析。结果显示，附睾精子在0分钟时，精子运动性显示出较高的值（p 与对照组相比，所有剂量的DPP≤0.05）。30、60和120分钟后，运动率较高（p > 0.05），所有实验组均与对照组进行比较。关于速度性状（VCL和VAP）和轨迹参数（LIN，ALH，BCF），40 mg / mL和80 mg / mL的剂量显示出比对照最高的值。在射精的样品中，仅在80 mg / mL的剂量下0分钟时的运动速率有所改善，而DPP在孵育60分钟后的所有剂量（20、40、80 mg / mL）下均显示出保护作用。DPP可以在任何储存点提高20 mg / mL的精子速度（VCL和VAP）和精子轨迹（LIN，ALH，BCF）。总之，通过保护和改善精子活力参数，枣棕花粉的水提物是兔精子补充剂的合适补充剂。

• 强调

• DPP提取物的添加改善了附睾和射精的兔子精子的质量。

• 在NPE中补充80 mg / mL DPP的补充剂对附睾精子的动力学特性具有有益的作用。尽管如此，在TPE补充剂中，DPP 20 mg / mL足以改善射精兔精液的精子运动性状。