Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), an enveloped RNA virus transmits by droplet infection thus affects the respiratory system. Different genomes have been reported globally for SARS-CoV-2 with moderate level of mutation which makes it harder to combat the virus. Mutational profiling and the relevant evolutionary aspect of coronavirus proteins namely spike glycoprotein, membrane protein, envelope protein, nucleoprotein, ORF1ab, ORF3a, ORF6, ORF7a, ORF7b and ORF8 were studied by in silico experiments. Clustering of the protein sequences and calculation of residue relative abundance were done to get an idea about the protein conservancy as well as finding out some representative sequences for phylogenetic and ancestral reconstruction. By mutational profiling and mutation analysis, the effect of mutations on the protein stability and their functional implication were studied. This study indicates the mutational effect on the proteins and their relevance in evolution, which directs us towards a better understanding of these variations and diversification of SARS-CoV-2 for useful future therapeutic study and thus aid in designing therapeutic agents keeping the highly variable regions in mind.

严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 是一种包膜 RNA 病毒，通过飞沫感染传播，因此会影响呼吸系统。全球报告了 SARS-CoV-2 的不同基因组，具有中等水平的突变，这使得对抗病毒变得更加困难。通过计算机实验研究了冠状病毒蛋白的突变谱和相关进化方面，即刺突糖蛋白、膜蛋白、包膜蛋白、核蛋白、ORF1ab、ORF3a、ORF6、ORF7a、ORF7b 和 ORF8。进行蛋白质序列的聚类和残基相对丰度的计算，以了解蛋白质的保守性，并找出一些用于系统发育和祖先重建的代表性序列。通过突变分析和突变分析，研究了突变对蛋白质稳定性的影响及其功能意义。这项研究表明了对蛋白质的突变影响及其在进化中的相关性，这使我们能够更好地了解 SARS-CoV-2 的这些变异和多样化，以进行有用的未来治疗研究，从而有助于设计保持高度可变区域的治疗剂心里。