The floral scents of plants play a key role in plant reproduction through the communication between plants and pollinators. Aquilegia as a model species for studying evolution, however, there have been few studies on the floral scents and relationships between floral scents and pollination for Aquilegia taxa. In this study, three types of solid-phase micro-extraction (SPME) fiber coatings (DVB/PDMS, CAR/PDMS, DVB/CAR/PDMS) were evaluated for their performance in extracting volatile organic compounds (VOCs) from flowers of Aquilegia amurensis, which can contribute to the future studies of elucidating the role of floral scents in the pollination process. In total, 55 VOCs were identified, and among them, 50, 47 and 45 VOCs were extracted by the DVB/CAR/PDMS fiber, CAR/PDMS fiber and DVB/PDMS fibers, respectively. Only 30 VOCs were detected in A. japonica taxa. Furthermore, the relative contents of 8 VOCs were significant different (VIP > 1 and p < 0.05) between the A. amurensis and A. japonica. The results can be applied in new studies of the relationships between the chemical composition of floral scents and the processes of attraction of pollinator. It may provide new ideas for rapid evolution and frequent interspecific hybridization of Aquilegia.

中文翻译：

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SPME-GC-MS的优化及花香的特性耧粳稻和A.椴花

通过植物与传粉媒介之间的交流，植物的花香在植物繁殖中起着关键作用。quil藜作为研究进化的典范物种，但是，关于A葵的类群的花香以及花香与授粉之间的关系的研究很少。在这项研究中，评估了三种固相微萃取（SPME）纤维涂层（DVB / PDMS，CAR / PDMS，DVB / CAR / PDMS）在从quil木花中提取挥发性有机化合物（VOC）的性能。 amurensis，可以为阐明花香在授粉过程中的作用的未来研究做出贡献。总共鉴定出55种VOC，其中DVB / CAR / PDMS纤维，CAR / PDMS纤维和DVB / PDMS纤维分别提取了50种，47种和45种VOC。在A中仅检测到30种VOC。粳稻类群。此外，八种挥发性有机化合物的相对含量在A. amurensis和A. japonica之间有显着差异（VIP> 1和p <0.05）。该结果可用于花香的化学成分与授粉媒介吸引过程之间关系的新研究。它可能为Aquilegia的快速进化和频繁的种间杂交提供新的思路。