Bacterial genetic material can be horizontally transferred between microorganisms via outer membrane vesicles (OMVs) released by bacteria. Up to now, the application of vesicle-mediated horizontal transfer of “degrading genes” in environmental remediation has not been reported. In this study, the nirS gene from an aerobic denitrification bacterium, Pseudomonas stutzeri, was enclosed in a pET28a plasmid, transformed into Escherichia coli (E. coli) DH5α and expressed in E. coli BL21. The E. coli DH5α released OMVs containing the recombination plasmid pET28a–nirS-EGFP. When compared with the free pET28a–nirS-EGFP plasmid’s inability to transform, nirS in OMVs could be transferred into E. coli BL21 with the transformation frequency of 2.76 × 10⁶ CFU/g when the dosage of OMVs was 200 µg under natural conditions, and nirS could express successfully in recipient bacteria. Furthermore, the recipient bacteria that received OMVs containing pET28a–nirS-EGFP could produce 18.16 U/mL activity of nitrite reductase.

细菌遗传物质可以通过细菌释放的外膜囊泡（OMV）在微生物之间水平转移。迄今为止，还没有报道过囊泡介导的“降解基因”水平转移在环境修复中的应用。在这项研究中，NIRS从好氧反硝化细菌基因，施氏假单胞菌，被封闭在pET28a中的质粒，转化到大肠杆菌（大肠杆菌）DH5α和在表达大肠杆菌BL21。该大肠杆菌含有重组DH5α释放OMV的质粒的pET28a NIRS -EGFP。与免费的pET28a– nirS相比-EGFP的质粒的无能变换，NIRS在OMV的可转移到大肠杆菌BL21与2.76转化频率×10 ⁶  CFU /克时的OMV的用量为在自然条件下200微克，和近红外光谱可以在受体细菌成功表达。此外，接受含有pET28a– nirS -EGFP的OMV的受体细菌可以产生18.16 U / mL的亚硝酸还原酶活性。