Research Progress and Prospects for Fructosyltransferases,ChemBioEng Reviews

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Research Progress and Prospects for Fructosyltransferases
ChemBioEng Reviews ( IF 6.2 ) Pub Date : 2021-04-22 , DOI: 10.1002/cben.202000011
Yitian Wang _{1,

2,

3} , Xiujie Shang _{1,

4} , Fan Cao ₅ , Haiquan Yang _{3,

6}

Affiliation

Fructosyltransferases can be used to produce fructooligosaccharides from sucrose and are obtained from different sources, mostly microorganisms and plants. Heterologous expression and several mutagenesis methods were applied to improve the production of fructosyltransferases. The structures and characteristics of fructosyltransferases were investigated. Immobilization and protein engineering can improve their characteristics. In this review, screening and mutagenesis of microorganisms, heterologous expression, purification, characterization, structural analysis, immobilization, and protein engineering of fructosyltransferases are discussed. Future prospects in this field are also considered.

中文翻译：

果糖基转移酶的研究进展与展望

果糖基转移酶可用于从蔗糖生产低聚果糖，并从不同来源获得，主要是微生物和植物。应用异源表达和几种诱变方法来提高果糖基转移酶的产生。研究了果糖基转移酶的结构和特性。固定化和蛋白质工程可以改善它们的特性。在这篇综述中，讨论了微生物的筛选和诱变、异源表达、纯化、表征、结构分析、固定化和果糖基转移酶的蛋白质工程。还考虑了该领域的未来前景。

更新日期：2021-06-15

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