Abstract

Background

Crohn's disease (CD) is a chronic inflammatory disease without a specific cause. Inflammation in these patients can disturb the oxidants/antioxidants balance and results in oxidative stress that plays a destructive role. This study aimed to evaluate the gene expression of sod1, sod2, cat, nrf2 and gp91phox in CD patients before and after Azathioprine (Aza) consumption.

Method

Peripheral bloodmononuclear cells (PBMCs) were separated from CD patients (n= 15, mean age = 33.6 ± 1.8) before and after treatment with Aza and healthy controls (n= 15, mean age = 31.5 ± 1.2). The expression levels of sod1, sod2, cat, nrf2 and gp91phox were measured in byusing real-time qRT-PCR technique.

Result

The expression levels of gp91phox (P-value < 0.001), cat (P-value < 0.05), sod1 (P-value < 0.001), nrf2 (P-value < 0.001) were significantly increased compared to control group. Following treatment with Aza, the decreased expression levels of gp91phox (P-value < 0.05), cat (P-value < 0.05), sod1(P-value < 0.001) and nrf2 (P-value < 0.001) were observed in CD patients.

Conclusion

Overall, our results showed that prescription of Azathioprine can lead to the altered expression of redox system-related genes in patients with CD.

中文翻译：

---

硫唑嘌呤可调节克罗恩病患者的氧化还原失衡

摘要

背景

克罗恩病 (CD) 是一种没有特定原因的慢性炎症性疾病。这些患者的炎症会扰乱氧化剂/抗氧化剂的平衡，并导致氧化应激，起到破坏性作用。本研究旨在评估CD 患者服用硫唑嘌呤 (Aza) 前后sod1、sod2、cat、nrf2和gp91phox的基因表达。

方法

在用 Aza 和健康对照（ n = 15，平均年龄 = 31.5 ± 1.2）治疗前后，从 CD 患者（ n = 15，平均年龄 = 33.6 ± 1.8）中分离外周血单个核细胞（PBMC ）。采用实时定量RT-PCR技术测定sod1、sod2、cat、nrf2和gp91phox的表达水平。

结果

与对照组相比，gp91phox（P值<0.001）、cat（P值<0.05）、sod1（P值<0.001）、nrf2（P值<0.001）的表达水平显着升高。在用 Aza 治疗后，在 CD 患者中观察到gp91phox ( P值 < 0.05)、cat ( P值 < 0.05)、sod1 ( P值 < 0.001) 和nrf2 ( P值 < 0.001) 的表达水平降低.

结论

总体而言，我们的研究结果表明，硫唑嘌呤的处方可导致 CD 患者氧化还原系统相关基因的表达发生改变。