Improving plant resistance against Fusarium wilt (FW) is key to sustaining chickpea production worldwide. Given this, the current study tested a set of 75 FW-responsive chickpea breeding lines including checks in a wilt-sick plot for two consecutive years (2016 and 2017). Genetic diversity analysis using 75 simple sequence repeats (SSRs) revealed a total of 267 alleles with an average of 3.56 alleles per marker. The entire set was divided into two major classes based on clustering method and factorial analysis. Similarly, STRUCTURE analysis placed the 75 genotypes into three distinct sub-groups (K = 3). Marker-trait association (MTA) analysis using the generalized linear model approach revealed nine and eight significant MTAs for FW resistance in the years 2016 and 2017, respectively. Three significant MTAs were obtained for FW resistance following the mixed linear model approach for both years. The SSR markers CESSR433, NCPGR21 and ICCM0284 could be potentially employed for targeted and accelerated improvement of FW resistance in chickpea. To the best of our knowledge, this is the first report on association mapping of the genomic loci controlling FW (Foc2) resistance in chickpea.

中文翻译：

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鹰嘴豆中与枯萎病抗性 (Foc2) 相关的基因组位点的关联作图

提高植物对枯萎病 (FW) 的抗性是维持全球鹰嘴豆生产的关键。鉴于此，目前的研究测试了一组 75 个 FW 响应鹰嘴豆育种系，包括连续两年（2016 年和 2017 年）在枯萎病区进行检查。使用 75 个简单序列重复 (SSR) 的遗传多样性分析揭示了总共 267 个等位基因，每个标记平均有 3.56 个等位基因。基于聚类方法和因子分析将整个集合分为两大类。同样，STRUCTURE 分析将 75 种基因型分为三个不同的亚组（ķ= 3)。使用广义线性模型方法进行的标记-性状关联 (MTA) 分析分别揭示了 2016 年和 2017 年有 9 个和 8 个显着的 FW 抗性 MTA。在两年的混合线性模型方法之后，获得了三个显着的 FW 阻力 MTA。SSR 标记 CESSR433、NCPGR21 和 ICCM0284 可潜在地用于靶向和加速改善鹰嘴豆的 FW 抗性。据我们所知，这是第一份关于控制 FW 的基因组位点关联作图的报告。焦点2) 鹰嘴豆的抗性。