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DNA barcoding of important fruit tree species of agronomic interest in the genus Garcinia L. from the Western Ghats
Genetic Resources and Crop Evolution ( IF 1.6 ) Pub Date : 2021-04-21 , DOI: 10.1007/s10722-021-01177-6
Jayesh Anerao , Vikas Jha , Nishaat Shaikh , Apurva Shivalkar , Aishwarya Nityanand , Dishanand Sawant , G. Ramachandra Rao , Kiran Mangaonkar , Manjushri Deodhar , Nitin Desai

Garcinia L. is a pantropically distributed genus with high species richness in South East Asia. It is a tropical evergreen plant with distinct morphological characteristics and has a high degree of endemism. Outstanding features of the Garcinia L. genus are monopodial growth, leafy texture, oil cavities containing yellow or light-colored resins present on all parts of the plant and polygamodioecious reproductive behavior. The current study was conducted to develop barcodes for different species of the genus Garcinia L., distributed widely in the Western Ghats of India. We assess the discrimination power of the plant DNA barcode (rbcL, matK, trnH-psbA, rpoB-trnCGAR and ITS), across major Garcinia L. species. Our results clearly demonstrate the value of plastid barcode data, previously unavailable for Garcinia L. species. Ten Garcinia L. species and three outgroup taxa selected from the Western Ghats of India for evaluation using four regions in the plastid genome (rbcL matK, trnH-psbA, rpoB-trnCGAR) and nuclear-transcribed spacer (nrITS) in order to discriminate them at the species level. A characteristic feature of all barcodes, maximum likelihood analysis, and Wilcoxon signed-rank test were used for species discrimination. The number of conserved sites were more using matK primer whereas more variables and informative sites found in rpoB-trnCGAR loci. For internal branches of species-specific clusters, maximum likelihood analysis showed a more resolved topology. Wilcoxon signed-rank test indicated a higher divergence for coding and non-coding regions. DNA barcoding was found to be a practical and rapid method for identifying more endemic species. These findings will potentially be helpful in delineating the various species of Garcinia L.



中文翻译:

西高止山脉属藤黄属中重要的具有农艺意义的果树种的DNA条形码

藤黄属(Garcinia L.)是一个分布在东南亚的具有高物种丰富度的泛热带属。它是一种热带常绿植物,具有独特的形态特征,并具有高度的特有性。藤黄属植物的突出特征是单足类生长,叶状纹理,植物的所有部位均存在含有黄色或浅色树脂的油腔以及一夫多妻制生殖行为。进行了当前的研究以开发针对藤黄属的不同物种的条形码,该条形码广泛分布在印度的西高止山脉。我们评估了主要藤黄植物DNA条形码(rbcL,matK,trnH-psbA,rpoB-trnCGAR和ITS)的辨别力L.种。我们的结果清楚地证明了质体条形码数据的价值,以前对于藤黄属物种不可用。十从印度西高止山脉中选出的L.物种和三个外群分类单元,使用质体基因组中的四个区域(rbcL matK,trnH-psbA,rpoB-trnCGAR)和核转录间隔区(nrITS)进行评估,以便在基因组中对其进行区分。物种级别。所有条形码的特征,最大似然分析和Wilcoxon符号秩检验用于物种识别。使用matK引物的保守位点数量更多,而在rpoB-trnCGAR基因座中发现了更多的变量和信息位点。对于特定物种集群的内部分支,最大似然分析显示了更解析的拓扑。Wilcoxon符号秩检验表明,编码区和非编码区的差异较大。发现DNA条形码是识别更多地方物种的实用而快速的方法。藤黄L.

更新日期:2021-04-21
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