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Evaluation of red-colored carotenoids in yeasts by the biomass color
Folia Microbiologica ( IF 2.4 ) Pub Date : 2021-04-21 , DOI: 10.1007/s12223-021-00871-3
Yanina Delegan 1 , Alena Yachkula 1 , Tatiana Antipova 1 , Mikhail Vainshtein 1
Affiliation  

Production of carotenoids with red yeasts is a promising area of industrial biotechnology. All spectrophotometrical (“classic”) analyses of carotenoids are based on preliminary extraction of the water-insoluble carotenoids; thus, these analyses are precise but complicated and time consuming. This paper presents a simple method to evaluate the red-colored carotenoids in yeast biomass by its color, without extraction. The method is based on digital characteristics of the biomass whole coloring, and it has already been successfully applied in other areas of biology: to compare plant and animal objects. In contrast to spectrophotometry measuring the amount of light that can pass through a solution, the biomass photo is a reflected color of the insoluble compounds. Application of this method to microorganisms permitted to compare the yeast strains and the effects of substrates or culturing regimes for any change in the red-colored pigments. The proposed rapid method was compared with the classic analyses of the carotenoids and showed that evaluation of red-colored carotenoids by the whole coloring of biomass can be used to discover changes in the yeast carotenoid production. In whole, the paper contributes method which is new for pigmented microorganisms and has a potential application in biotechnology.



中文翻译:

通过生物量颜色评价酵母中红色类胡萝卜素

用红酵母生产类胡萝卜素是工业生物技术的一个有前途的领域。类胡萝卜素的所有分光光度法(“经典”)分析均基于对水不溶性类胡萝卜素的初步提取;因此,这些分析是精确但复杂且耗时的。本文提出了一种无需提取即可通过颜色评估酵母生物质中红色类胡萝卜素的简单方法。该方法基于生物质整体着色的数字特征,已成功应用于生物学的其他领域:植物和动物物体的比较。与测量可通过溶液的光量的分光光度法相比,生物质照片是不溶性化合物的反射颜色。将此方法应用于微生物,允许比较酵母菌株和底物或培养方案对红色色素的任何变化的影响。将所提出的快速方法与类胡萝卜素的经典分析进行比较,并表明通过生物质的整体着色评估红色类胡萝卜素可用于发现酵母类胡萝卜素产量的变化。总体而言,该论文为有色微生物提供了一种新的方法,在生物技术中具有潜在的应用价值。将所提出的快速方法与类胡萝卜素的经典分析进行比较,并表明通过生物质的整体着色评估红色类胡萝卜素可用于发现酵母类胡萝卜素产量的变化。总体而言,该论文为有色微生物提供了一种新的方法,在生物技术中具有潜在的应用价值。将所提出的快速方法与类胡萝卜素的经典分析进行比较,并表明通过生物质的整体着色评估红色类胡萝卜素可用于发现酵母类胡萝卜素产量的变化。总体而言,该论文为有色微生物提供了一种新的方法,在生物技术中具有潜在的应用价值。

更新日期:2021-04-21
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