Abstract

This study aimed to investigate the dietary mineral and vitamin supplementation on the hematological, the circulating biochemical parameters, and the reproductive performance of cyclic mares aged 4–12 years old. Purebred Arabian (PAB, n = 8) and East-Bulgarian (EBB, n = 12) breeds were equally divided into treated and control groups. The nutritional supplement was given orally (50 g daily/animal/day) for seven consecutive days and repeated for 4 consecutive months. All mares were examined daily with ultrasound from exhibiting oestrus signs till ovulation. Blood samples were collected with and without anticoagulant before supplementation, on Day 7 and Day 120 for the measurement of ALT, lactate dehydrogenase, bilirubin, calcium, phosphorus, and creatinine and to perform full blood picture. The nutritional supplement decreased lactate dehydrogenase (p < .05) and creatinine (p < .05). Compared to the PAB mares, control mares had one day shorter oestrous cycle length (20.2 ± 2.13 vs 21.0 ± 2.46 days), but the treated EBB mares had two days shorter oestrus cycle (20.00 ± 1.202 vs 22.00 ± 0.93 days). The oestrus phase of the treated mares was shorter (p < .001) than that of the control mares (3.63 ± 0.38 vs 5.50 ± 0.31 days). The diameter of the dominant follicle varied (p < .05) between the two breeds that was 38.25 ± 1.90 and 36.94 ± 1.70 mm for treated and control EBB but was 35.38 ± 1.41 and 34.89 ± 1.35 mm for treated and control PAB. In conclusion, the nutritional supplement had a good effect on the reproductive status, and on the blood parameters (hematological and biochemical) of the mares, but more research is necessary to recommend its use.

• Highlights

• The addition of the nutritional supplementation to the mares from the East-Bulgarian breed lead to shorter oestrus.

• There was a tendency to shorten the sexual cycle (up to 2 days) and increase the size of the dominant follicles in mares receiving the supplement.

摘要

这项研究旨在研究膳食矿物质和维生素补充对4至12岁循环母马的血液学，循环生化参数和生殖性能的影响。纯种阿拉伯（PAB，n  = 8）和东保加利亚（EBB，n = 12）将品种平均分为治疗组和对照组。连续7天口服（每天/动物/每天50 g）营养补充剂，并连续4个月重复。从出现发情迹象到排卵，每天对所有母马进行超声波检查。在补充之前和不补充抗凝剂的情况下，在第7天和第120天收集血液样本，以测量ALT，乳酸脱氢酶，胆红素，钙，磷和肌酐，并进行全血检查。营养补充剂可降低乳酸脱氢酶（p <  .05）和肌酐（p < .05）。与PAB母马相比，​​对照母马的发情周期长度短了一天（20.2±2.13 vs 21.0±2.46天），但是处理过的EBB母马的发情周期短了两天（20.00±1.202 vs 22.00±0.93天）。处理过的母马的发情期比对照母马的发情期短（p <  .001）（3.63±0.38 vs 5.50±0.31天）。 两个品种之间的优势卵泡直径变化（p < .05），经处理和对照的EBB分别为38.25±1.90和36.94±1.70 mm，而经处理和对照的PAB分别为35.38±1.41和34.89±1.35 mm。总之，营养补充剂对母马的繁殖状况和血液参数（血液学和生化指标）都有很好的作用，但是需要更多的研究来推荐其用途。

• 强调

• 在东保加利亚品种的母马中添加营养补充剂会导致发情期缩短。

• 在接受补充剂的母马中，有一种缩短性周期（长达2天）并增加优势卵泡大小的趋势。