Herein, we report the development of a two-dimensional (2D) label-free matrix for cardiac biomarker detection. Tungsten trioxide nanosheets (WO₃ NS) were synthesized by a facile, environmentally friendly hydrothermal method and subsequently functionalized chemically using 3-aminopropyletriethoxysilane (APTES). Thin film of APTES functionalized WO₃ NS was deposited on indium tin oxide (ITO) substrate using electrophoretic deposition technique. The fabricated APTES/WO₃ NS/ITO electrode endows with large surface area for high loading of cardiac troponin I antibodies (anti-cTnI), good stability and strong adsorption platform for the biomolecules immobilization. The selective and quantitative detection of cTnI biomarker was accomplished by immobilizing anti-cTnI on APTES/WO₃ NS/ITO platform. The electrochemical sensing using anti-cTnI/APTES/WO₃ NS/ITO immunoelectrode was studied as a label-free immunoassay format using EIS (electrochemical impedance spectroscopy) technique. The performance of the sensor as investigated with the measurement of charge transfer resistance (R_CT) for variant concentrations of cTnI antigen demonstrated good sensitivity as 30.8 Ω mL ng⁻¹ cm⁻² for a linear detection range 0.1 to 100 ng mL⁻¹ along with good stability up to 6 weeks. The competency of the biosensor was also tested by detecting cTnI antigen in spiked human serum samples.

在此，我们报告了用于心脏生物标志物检测的二维（2D）无标签基质的开发。三氧化钨纳米片（WO ₃ NS）通过一种简便的，环境友好的水热法合成，随后使用3-氨基丙基乙氧基硅烷（APTES）进行化学功能化。使用电泳沉积技术，将APTES功能化WO ₃ NS薄膜沉积在氧化铟锡（ITO）衬底上。人造APTES / WO ₃NS / ITO电极具有较大的表面积，可高负载心肌肌钙蛋白I抗体（抗cTnI），良好的稳定性和强大的生物分子固定平台。通过将抗cTnI固定在APTES / WO ₃ NS / ITO平台上来完成cTnI生物标志物的选择性和定量检测。研究了使用抗cTnI / APTES / WO ₃ NS / ITO免疫电极进行的电化学感应，它是使用EIS（电化学阻抗谱）技术的无标记免疫分析形式。在不同浓度的cTnI抗原的电荷转移电阻（R _CT）测量中研究的传感器性能表现出良好的灵敏度，为30.8ΩmL ng ^-1  cm ^-2线性检测范围为0.1到100 ng mL ^-1，并具有长达6周的良好稳定性。还通过检测加标的人血清样品中的cTnI抗原来测试生物传感器的能力。