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Single-walled carbon nanotube-gold urchin nanohybrid for identifying gastric cancer on dimicroelectrodes junction
Journal of the Taiwan Institute of Chemical Engineers ( IF 5.5 ) Pub Date : 2021-04-21 , DOI: 10.1016/j.jtice.2021.04.016
Zhihong Yu , Subash C.B. Gopinath , Thangavel Lakshmipriya , Periasamy Anbu

Diagnosing gastric cancer with a high accuracy helps in identifying at early stage and for a better treatment. A transcription factor, SRY-box containing gene-17 (SOX-17) shows a crucial role in the gastric cancer progression is desired as biomarker on a high-performance sensing platform, dimicroelectrodes Junction (DMEJ), constructed by the hybrid of single-walled carbon nanotube (SWCN) and gold urchin (GU) surface to detect the specific SOX-17 target oligonucleotide. SWCN was modified on DMEJ and GU conjugated capture DNA was immobilized on SWCN through the electrostatic interaction of GU by (3-Aminopropyl)triethoxysilane. Morphological analyses by FESEM and FETEM revealed the attachment of GU on SWCN with a uniform fashion. SWCN-GU modified DMEJ has enhanced an ideal electric flow and reflecting the capture DNA attachment. Reached the limit of detection at 1 aM, calculated on a linear curve (atto- to femto-molar) with the regression coefficient of R2 = 0.9909[y = 1.1226x + 1.3727]. Further, specificity of SOX-17 was confirmed by interacting complementary, single- and five-mismatched sequences of the target. In addition, the target DNA solution mixed human serum and control sequences did not interfere with the specific duplex formation, indicating the non-biofouling on DMEJ surface. This SWCN-GU constructed DMEJ surface helps to identify lower expression of SOX-17 and diagnosing gastric cancer progression.



中文翻译:

单壁碳纳米管-金顽童纳米杂交体在双微电极连接处鉴定胃癌

高度准确地诊断胃癌有助于早期识别并获得更好的治疗。转录因子SRY-box含有基因17(SOX-17),在胃癌的进展中起关键作用。作为高性能的传感平台,dimicroelectrodes Junction(DMEJ)上的生物标志物是人们所希望的。壁碳纳米管(SWCN)和金顽童(GU)表面,以检测特定的SOX-17目标寡核苷酸。在DMEJ上修饰SWCN,并通过(3-氨基丙基)三乙氧基硅烷与GU的静电相互作用,将GU共轭的捕获DNA固定在SWCN上。FESEM和FETEM的形态分析表明,GU以均匀的方式附着在SWCN上。SWCN-GU修饰的DMEJ增强了理想的电流并反映了捕获的DNA附着。2  = 0.9909 [ y  = 1.1226 x  + 1.3727]。此外,通过与靶标的互补,单和五错配序列相互作用,证实了SOX-17的特异性。此外,目标DNA溶液混合人血清和对照序列不会干扰特异性双链体的形成,表明DMEJ表面无生物结垢。这种SWCN-GU构造的DMEJ表面有助于鉴定SOX-17的较低表达并诊断胃癌的进展。

更新日期:2021-05-05
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