Diagnosing gastric cancer with a high accuracy helps in identifying at early stage and for a better treatment. A transcription factor, SRY-box containing gene-17 (SOX-17) shows a crucial role in the gastric cancer progression is desired as biomarker on a high-performance sensing platform, dimicroelectrodes Junction (DMEJ), constructed by the hybrid of single-walled carbon nanotube (SWCN) and gold urchin (GU) surface to detect the specific SOX-17 target oligonucleotide. SWCN was modified on DMEJ and GU conjugated capture DNA was immobilized on SWCN through the electrostatic interaction of GU by (3-Aminopropyl)triethoxysilane. Morphological analyses by FESEM and FETEM revealed the attachment of GU on SWCN with a uniform fashion. SWCN-GU modified DMEJ has enhanced an ideal electric flow and reflecting the capture DNA attachment. Reached the limit of detection at 1 aM, calculated on a linear curve (atto- to femto-molar) with the regression coefficient of R² = 0.9909[y = 1.1226x + 1.3727]. Further, specificity of SOX-17 was confirmed by interacting complementary, single- and five-mismatched sequences of the target. In addition, the target DNA solution mixed human serum and control sequences did not interfere with the specific duplex formation, indicating the non-biofouling on DMEJ surface. This SWCN-GU constructed DMEJ surface helps to identify lower expression of SOX-17 and diagnosing gastric cancer progression.

高度准确地诊断胃癌有助于早期识别并获得更好的治疗。转录因子SRY-box含有基因17（SOX-17），在胃癌的进展中起关键作用。作为高性能的传感平台，dimicroelectrodes Junction（DMEJ）上的生物标志物是人们所希望的。壁碳纳米管（SWCN）和金顽童（GU）表面，以检测特定的SOX-17目标寡核苷酸。在DMEJ上修饰SWCN，并通过（3-氨基丙基）三乙氧基硅烷与GU的静电相互作用，将GU共轭的捕获DNA固定在SWCN上。FESEM和FETEM的形态分析表明，GU以均匀的方式附着在SWCN上。SWCN-GU修饰的DMEJ增强了理想的电流并反映了捕获的DNA附着。²  = 0.9909 [ y  = 1.1226 x  + 1.3727]。此外，通过与靶标的互补，单和五错配序列相互作用，证实了SOX-17的特异性。此外，目标DNA溶液混合人血清和对照序列不会干扰特异性双链体的形成，表明DMEJ表面无生物结垢。这种SWCN-GU构造的DMEJ表面有助于鉴定SOX-17的较低表达并诊断胃癌的进展。