CD133 is a valuable prognostic marker in multiple types of cancer. However, the expression, methylation levels, and prognostic relevance of CD133 have not been evaluated in a pan-cancer perspective. The expression and methylation levels of CD133 across different types of cancer were determined using The Cancer Genome Atlas (TCGA) dataset. Univariate cox regression and Kaplan-Meier survival were used to determine the prognostic significance of CD133 expression and methylation. CD133 was highly expressed in papillary renal cell carcinoma (PRCC) or pancreatic adenocarcinoma (PAAD). Correspondingly, PAAD and PRCC had low CD133 methylation levels. Through pan-cancer perspective analysis, we found that CD133 high expression was a poor prognostic factor in lower grade glioma (LGG), while, CD133 high expression was a good prognostic factor in PRCC. Moreover, genes positively correlated with CD133 expression were associated with the poor clinical outcomes of LGG. In PRCC, genes negatively correlated with CD133 expression were correlated with the poor overall survival. Furthermore, CD133 expression levels were highly correlated with the CD133 methylation levels in LGG or PRCC. Correspondingly, CD133 hypermethylation was a good prognostic factor in LGG. On the contrary, CD133 hypomethylation was a good prognostic factor in PRCC. We also found that CD133 was highly expressed and hypomethylated in wild type IDH subgroup of LGG. CD133 was highly expressed and hypomethylated in low stages and type1 of PRCC. CD133 high expression and hypomethylation were bad prognostic factors in LGG, while, CD133 high expression and hypomethylation were good prognostic factors in PRCC.

CD133 是多种癌症的重要预后标志物。然而，CD133 的表达、甲基化水平和预后相关性尚未从泛癌角度进行评估。使用癌症基因组图谱 (TCGA) 数据集确定不同类型癌症中 CD133 的表达和甲基化水平。使用单变量 cox 回归和 Kaplan-Meier 生存来确定 CD133 表达和甲基化的预后意义。 CD133 在乳头状肾细胞癌 (PRCC) 或胰腺腺癌 (PAAD) 中高表达。相应地，PAAD 和 PRCC 的 CD133 甲基化水平较低。通过泛癌视角分析，我们发现CD133高表达是低级别胶质瘤（LGG）的不良预后因素，而CD133高表达是PRCC的良好预后因素。此外，与 CD133 表达正相关的基因与 LGG 不良的临床结果相关。在 PRCC 中，与 CD133 表达负相关的基因与较差的总生存率相关。此外，CD133 表达水平与 LGG 或 PRCC 中的 CD133 甲基化水平高度相关。相应地，CD133 高甲基化是 LGG 的良好预后因素。相反，CD133 低甲基化是 PRCC 的良好预后因素。我们还发现 CD133 在 LGG 野生型 IDH 亚群中高表达且低甲基化。 CD133 在 PRCC 的低期和 1 型中高表达且低甲基化。 CD133高表达和低甲基化是LGG的不良预后因素，而CD133高表达和低甲基化是PRCC的良好预后因素。