This article describes the fungi isolated from the blighted leaf of rubber (Hevea brasiliensis) tree in Malaysia. Previously, Neofusicoccum ribis (Family Botryosphaeriaceae) was reported as the causal agent of rubber leaf blight in Malaysia. To date, there are limited studies on this particular disease in Malaysia and worldwide. The current study was carried out on rubber leaves displaying leaf blight symptoms caused by N. ribis from different locations to discover the fungi that reside in the blighted leaves. Rubber leaf samples with blight symptoms were collected from two different locations in Malaysia. The fungal isolates were isolated following the agar plate method and subjected to cultural and morphological characterization. Based on the cultural and morphological characterization, eight fungal isolates were further subjected to molecular identification. Five fungal isolates namely SBI, SB2, SB3, SB6 and SPKT1 were identified as N. ribis, one fungus (SB4) as Diaporthe drenthii, and another two fungi namely SB5 and SPKT2 were identified as Lasiodiplodia pseudotheobromae. Based on the morphological and molecular identification, all the N. ribis isolates demonstrated similar characteristics of the rubber leaf blight pathogen as documented in a previous study.

中文翻译：

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与马来西亚橡胶树枯叶症状相关的真菌

本文介绍了从马来西亚橡胶树（巴西橡胶树）枯萎的叶子中分离出的真菌。此前，Neofusicoccum ribis (Family Botryosphaeriaceae) 被报道为马来西亚橡胶叶枯病的病原体。迄今为止，在马来西亚和世界范围内对这种特定疾病的研究有限。目前的研究是对显示由N引起的叶枯病症状的橡胶叶进行的。 肋骨从不同的地方发现枯萎的叶子中的真菌。从马来西亚的两个不同地点收集了具有枯萎病症状的橡胶叶样本。按照琼脂平板法分离真菌分离株并进行培养和形态学表征。基于培养和形态特征，进一步对八株真菌进行了分子鉴定。五层真菌分离，即SBI，SB2，SB3，SB6和SPKT1被认定为ñ。 ribis，一种真菌 (SB4) 为Diaporthe drenthii，另外两种真菌即 SB5 和 SPKT2 被鉴定为Lasiodiplodiapseudotheobromae。基于形态学和分子鉴定，所有的N. ribis分离株表现出与先前研究中记录的橡胶叶枯病病原体相似的特征。