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Development of mycoinsecticide formulations with Beauveria bassiana and Metarhizium brunneum for the control of Orosanga japonica (Hemiptera: Ricaniidae)
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2021-04-20 , DOI: 10.1111/aab.12699 Seda Biryol 1 , Ebru Güney 1 , Ardahan Eski 2, 3 , Zeynep Bayramoğlu 4 , Kazım Sezen 1 , Zihni Demirbag 1 , İsmail Demir 1
Annals of Applied Biology ( IF 2.2 ) Pub Date : 2021-04-20 , DOI: 10.1111/aab.12699 Seda Biryol 1 , Ebru Güney 1 , Ardahan Eski 2, 3 , Zeynep Bayramoğlu 4 , Kazım Sezen 1 , Zihni Demirbag 1 , İsmail Demir 1
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Entomopathogenic fungi are promising microbial agents for agricultural pests, especially piercing-sucking insects. In this study, eight indigenous fungal isolates including four isolates of Metarhizium brunneum and four isolates of Beauveria bassiana were tested on Orosanga japonica Melichar (Hemiptera: Ricaniidae) in order to find an effective control agent that can be utilised for the development of oil-based fungal mycoinsecticides. In the screening test using 1 × 107 spores ml−1 concentration, KTU-24 (B. bassiana) and KTU-51 (M. brunneum) had the highest lethal activity against nymphs and adults of the target pest. Also, at the highest concentration (109 spores ml−1), KTU-24 showed 92.33 and 94.88% mortality on nymphs and adults at the dose–response tests, respectively, and the KTU-51 isolate showed 100% mortality on both stages of O. japonica under laboratory conditions. With these results, KTU-24 and KTU-51 isolates were used to develop biopesticides. While the mass production of B. bassiana was carried out with liquid-state fermentation, solid-state fermentation was used for mass production of M. brunneum. Spores of both isolates were formulated in oil, and products were named as RICANICIDAL Bbas-TR61 and RICANICIDAL Met-TR61, respectively. Formulations caused over 97% mortality against nymphs and adults of O. japonica under field conditions and both formulations showed higher efficacy than commercial mycoinsecticides at 108 spores/ml concentration 20 days after treatment. The currents study shows that both mycoinsecticide formulations are highly promising for management of O. japonica.
中文翻译：
开发含有白僵菌和绿僵菌的杀真菌剂制剂，用于控制粳稻（半翅目：Ricaniidae）
昆虫病原真菌是农业害虫，特别是刺吸昆虫的有前途的微生物制剂。在这项研究中，在Orosanga japonica Melichar（半翅目：Ricaniidae）上测试了8 株本地真菌，包括 4 株绿僵菌和 4 株白僵菌，以寻找可用于开发油基真菌的有效控制剂。真菌杀真菌剂。在使用1×10 7孢子ml -1浓度的筛选试验中，KTU-24 ( B . bassiana ) 和 KTU-51 ( M . brunneum) 对目标害虫的若虫和成虫具有最高的致死活性。此外，在最高浓度（10 9孢子 ml -1）下，KTU-24 在剂量反应测试中对若虫和成虫的死亡率分别为 92.33% 和 94.88%，而 KTU-51 分离株在两个阶段均显示出 100% 的死亡率的Ø。粳稻在实验室条件下。根据这些结果，KTU-24 和 KTU-51 分离株被用于开发生物农药。而大量生产B . 白僵菌用液态发酵，用于大规模生产的固态发酵进行中号。布鲁内姆. 两种分离物的孢子均在油中配制，产品分别命名为 RICANICIDAL Bbas -TR61 和 RICANICIDAL Met -TR61。制剂对O 的若虫和成虫造成超过 97% 的死亡率。japonica在田间条件下和两种制剂在处理后 20 天以 10 8孢子/毫升浓度显示出比商业杀真菌剂更高的功效。目前的研究表明，这两种杀真菌剂制剂在控制O方面都非常有希望。粳稻。
更新日期：2021-04-20
中文翻译：
开发含有白僵菌和绿僵菌的杀真菌剂制剂，用于控制粳稻（半翅目：Ricaniidae）
昆虫病原真菌是农业害虫，特别是刺吸昆虫的有前途的微生物制剂。在这项研究中，在Orosanga japonica Melichar（半翅目：Ricaniidae）上测试了8 株本地真菌，包括 4 株绿僵菌和 4 株白僵菌，以寻找可用于开发油基真菌的有效控制剂。真菌杀真菌剂。在使用1×10 7孢子ml -1浓度的筛选试验中，KTU-24 ( B . bassiana ) 和 KTU-51 ( M . brunneum) 对目标害虫的若虫和成虫具有最高的致死活性。此外，在最高浓度（10 9孢子 ml -1）下，KTU-24 在剂量反应测试中对若虫和成虫的死亡率分别为 92.33% 和 94.88%，而 KTU-51 分离株在两个阶段均显示出 100% 的死亡率的Ø。粳稻在实验室条件下。根据这些结果，KTU-24 和 KTU-51 分离株被用于开发生物农药。而大量生产B . 白僵菌用液态发酵，用于大规模生产的固态发酵进行中号。布鲁内姆. 两种分离物的孢子均在油中配制，产品分别命名为 RICANICIDAL Bbas -TR61 和 RICANICIDAL Met -TR61。制剂对O 的若虫和成虫造成超过 97% 的死亡率。japonica在田间条件下和两种制剂在处理后 20 天以 10 8孢子/毫升浓度显示出比商业杀真菌剂更高的功效。目前的研究表明，这两种杀真菌剂制剂在控制O方面都非常有希望。粳稻。
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