Transcription factor, skinhead-1 (skn-1) has been demonstrated to play central roles in regulation of oxidative damage. Arsenite is an oxidative damage inducer in the environment. However, the role of skn-1 in arsenite-induced oxidative damage remains unclear. Thus, in this study, by using RNAi feeding, different toxic responses of wild-type and skn-1 knockdown nematodes to arsenite were evaluated. Our results demonstrated that arsenite did not show any significant impacts on locomotory behaviors, but skn-1 knock-down worms were much more sensitive to arsenite treatment, manifested by an aggravated reduction of survival rate than that of wild-type nematodes. In arsenite-treated worms, down-regulation of skn-1 significantly exacerbated the arsenite-induced changed expressions of oxidative damage-related genes, xbp-1, apl-1 and trxr-2, but these regulated effects of skn-1 were not observed on spr-4 and sel-12 expressions under arsenite treatment. These findings together suggest that skn-1 may play a vital role in protection of C. elegans from arsenite-induced oxidative damage.

转录因子，光头 1 ( skn-1 ) 已被证明在调节氧化损伤中起重要作用。亚砷酸盐是环境中的氧化损伤诱导剂。然而，skn-1在亚砷酸盐诱导的氧化损伤中的作用仍不清楚。因此，在本研究中，通过使用 RNAi 喂养，评估了野生型和skn-1敲低线虫对亚砷酸盐的不同毒性反应。我们的研究结果表明，亚砷酸盐对运动行为没有任何显着影响，但skn-1敲除蠕虫对亚砷酸盐处理更为敏感，与野生型线虫相比，其存活率显着降低。在亚砷酸盐处理的蠕虫中， skn-1的下调显着加剧了亚砷酸盐诱导的氧化损伤相关基因xbp-1、apl-1和trxr-2的表达变化，但在亚砷酸盐处理下，未观察到skn-1对spr-4和sel-12表达的这些调节作用. 这些发现共同表明，skn-1可能在保护秀丽隐杆线虫免受亚砷酸盐诱导的氧化损伤方面发挥重要作用。