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The significance of genotypic diversity in coral competitive interaction: a transcriptomic perspective
Frontiers in Ecology and Evolution ( IF 2.4 ) Pub Date : 2021-04-21 , DOI: 10.3389/fevo.2021.659360
N. Andrade Rodriguez , A. Moya , R. Jones , D. J. Miller , I. R. Cooke

Competitive interactions shape coral assemblages and govern the dynamics of coral ecosystems. Although competition is an ecological concept the outcomes of competitive interactions are ultimately determined by patterns of gene expression. These patterns are subject to genotypic variation on both sides of any interaction. Such variation is typically treated as “noise”, but it is sometimes possible to identify patterns within it that reveal important hidden factors in an experiment. To incorporate genotypic variation into the investigation of coral competitive interactions, we used RNA-sequencing to study changes in gene expression in a hard coral (Porites cylindrica) resulting from non-contact competition experiment with a soft coral (Lobophytum pauciflorum). Hard coral genotype explained the largest proportion of variation between samples; however, it was also possible to detect gene expression changes in 76 transcripts resulting from interaction with the soft coral. In addition, we found a group of 20 short secreted proteins that were expressed as a coordinated unit in three interacting Porites-Lobophytum pairs. The presence of this secretion response was idiosyncratic in that it could not be predicted based on polyp behaviour, or the genotype of hard or soft coral alone. This study illustrates the significance of individual variation as a determinant of competitive behaviour, and also provides some intriguing glimpses into the molecular mechanisms employed by hard corals competing at a distance

中文翻译:

基因型多样性在珊瑚竞争相互作用中的意义:转录组学观点

竞争性相互作用塑造了珊瑚的组合并控制着珊瑚生态系统的动态。尽管竞争是一个生态概念,但是竞争相互作用的结果最终取决于基因表达的模式。这些模式在任何相互作用的两侧都受到基因型变异的影响。此类变化通常被视为“噪声”,但有时可以识别其中揭示了实验中重要隐藏因素的模式。为了将基因型变异纳入珊瑚竞争相互作用的研究中,我们使用RNA测序研究了硬珊瑚(Porites cylindrica)中的基因表达变化,这种变化是由与软珊瑚(Lobophytum pauciflorum)的非接触竞争实验产生的。硬珊瑚基因型解释了样本之间变异的最大比例;然而,还可以检测到与软珊瑚相互作用导致的76个转录本中的基因表达变化。此外,我们发现了一组20个短分泌蛋白,它们在三个相互作用的Porites-Lobophytum对中作为协调单元表达。这种分泌反应的存在是特异的,因为无法根据息肉的行为或单独的硬珊瑚或软珊瑚的基因型来预测它。这项研究说明了个体变异作为竞争行为的决定因素的重要性,并为硬珊瑚在远距离竞争中使用的分子机制提供了一些有趣的发现。我们发现了一组20个短分泌蛋白,它们在三个相互作用的Porites-Lobophytum对中作为协调单元表达。这种分泌反应的存在是特异的,因为无法根据息肉的行为或单独的硬珊瑚或软珊瑚的基因型来预测它。这项研究说明了个体变异作为竞争行为的决定因素的重要性,并为硬珊瑚在远距离竞争中使用的分子机制提供了一些有趣的发现。我们发现了一组20个短分泌蛋白,它们在三个相互作用的Porites-Lobophytum对中作为协调单元表达。这种分泌反应的存在是特异的,因为无法根据息肉的行为或单独的硬珊瑚或软珊瑚的基因型来预测它。这项研究说明了个体变异作为竞争行为的决定因素的重要性,并为硬珊瑚在远距离竞争中使用的分子机制提供了一些有趣的发现。
更新日期:2021-04-21
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