The ability to monitor oxygen delivery in microvasculature plays a vital role in measuring the viability of skin tissue and the probability of recovery. Using currently available clinical imaging tools, it is difficult to observe non-invasive hemodynamic regulation in the peripheral vessels. Here we propose the use of a novel multispectral raster-scanning optoacoustic mesoscopy (RSOM) system for noninvasive clinical monitoring of hemodynamic changes in the skin microvasculature’s oxy- (HbO₂) and deoxy-hemoglobin (Hb), total hemoglobin (HbT) and oxygen saturation (rsO₂). High resolution images of hemoglobin distribution in the skin microvasculature from six healthy volunteers during venous and arterial occlusion, simulating systemic vascular diseases are presented. During venous occlusion, Hb and HbO₂ optoacoustic signals showed an increasing trend with time, followed by a drop in the values after cuff deflation. During arterial occlusion, an increase in Hb value and decrease in HbO₂ values was observed, followed by a drop in Hb and jump in HbO₂ values after the cuff deflation. A decrease in rsO₂ values during both venous and arterial occlusion was observed with an increase in value after occlusion release. Using this proof of concept study, hereby we propose multispectral RSOM as a novel tool to measure high resolution hemodynamic changes in microvasculature for investigating systemic vascular diseases on peripheral tissues and also for monitoring inflammatory skin diseases, and its therapeutic interventions.

监测微脉管系统中氧气输送的能力在测量皮肤组织的活力和恢复的可能性中起着至关重要的作用。使用当前可用的临床成像工具，很难观察到外周血管的非侵入性血液动力学调节。在这里，我们建议使用新型多光谱光栅扫描光声内镜（RSOM）系统对皮肤微脉管中的氧-（HbO ₂）和脱氧-血红蛋白（Hb），总血红蛋白（HbT）和氧的血流动力学变化进行无创临床监测饱和度（rsO ₂）。呈现了六名健康志愿者在静脉和动脉闭塞过程中模拟系统性血管疾病的血红蛋白在皮肤微血管中分布的高分辨率图像。在静脉闭塞过程中，Hb和HbO _2的光声信号显示随时间增加的趋势，然后在袖带放气后其值下降。在动脉闭塞期间，观察到袖带放气后Hb值升高和HbO ₂值降低，随后是Hb下降和HbO ₂值跳跃。rsO ₂降低在静脉和动脉闭塞过程中观察到的值随着闭塞释放后的值增加而增加。利用这一概念验证研究，我们在此提出多光谱RSOM作为一种新工具，用于测量微脉管系统中的高分辨率血流动力学变化，以研究外周组织上的系统性血管疾病，还用于监测炎症性皮肤病及其治疗性干预措施。