New psychoactive substances are becoming increasingly popular. However, there is a lack of mass spectral information on parent substances and their corresponding metabolites as well as fatal concentrations in body liquids and tissues. Only very few clinical reports and user reports exist. This is also the case for methylaminopropylbenzofuran (MAPB) isomers. Urine from a decedent was screened using different immuno assays, gas chromatography-mass spectrometry (GC–MS), and liquid chromatography-high resolution mass spectrometry (LC-HRMS/MS). Additionally, respective targets were quantitated in all analyzed specimens by LC–MS/MS. Gas chromatography-infrared spectroscopy (GC-IR) was applied to confirm the identity of the intoxication. GC–MS and the amphetamine immuno assay were useful to track the putative intoxication. However, the chemical structure of 2-MAPB was only elucidated by means of LC-HRMS/MS and GC-IR. 2-MAPB was highest in urine (167 µg/mL), followed by gastric content (98.9 µg/mL), bile fluid (30.8 µg/mL), liver (22.2 µg/g), heart blood (16.7 µg/mL), and lowest in femoral blood (7.3 µg/mL). Besides the parent substance, we detected N-demethyl-2-MAPB and hydroxy-2-MAPB in the urine sample. This case report presents an intoxication caused by 2-MAPB. The 2-MAPB concentration found in femoral blood exceeded those reviewed for 5/6-MAPB. The concentrations of the other specimens cannot be evaluated because there exist no comparative data. The values presented can be applied to assess 2-MAPB intoxications in the future.

新的精神活性物质变得越来越受欢迎。但是，缺乏有关母体物质及其相应代谢产物以及体液和组织中致命浓度的质谱信息。仅存在很少的临床报告和用户报告。甲基氨基丙基苯并呋喃（MAPB）异构体也是如此。使用不同的免疫测定，气相色谱-质谱（GC-MS）和液相色谱-高分辨率质谱（LC-HRMS / MS）筛选死者的尿液。此外，通过LC-MS / MS在所有分析样品中定量了各自的目标。气相色谱-红外光谱法（GC-IR）用于确认中毒的身份。GC-MS和苯丙胺免疫分析法可用于追踪假定的中毒情况。然而，仅通过LC-HRMS / MS和GC-IR阐明了2-MAPB的化学结构。2-MAPB的尿液含量最高（167 µg / mL），其次是胃液含量（98.9 µg / mL），胆汁（30.8 µg / mL），肝脏（22.2 µg / g），心脏血液（16.7 µg / mL） ，并且在股血中最低（7.3 µg / mL）。除了母体物质，我们还检测到尿液样本中的N-脱甲基-2-MAPB和羟基-2-MAPB。该病例报告提出了由2-MAPB引起的中毒。股血中的2-MAPB浓度超过了5 / 6-MAPB的浓度。由于没有比较数据，因此无法评估其他样品的浓度。所提供的值可用于将来评估2-MAPB中毒。