Karyomorphological and SEM studies in newly discovered populations of a critically endangered medicinal plant — Lilium polyphyllum D. Don ex Royle from a high altitude protected area of north-western Himalaya,Microscopy Research and Technique

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Karyomorphological and SEM studies in newly discovered populations of a critically endangered medicinal plant — Lilium polyphyllum D. Don ex Royle from a high altitude protected area of north-western Himalaya
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-04-20 , DOI: 10.1002/jemt.23784
Puneet Kumar ₁ , Harminder Singh , Sushil Kumar Singh ₁

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In this study, new populations of a critically endangered Himalayan herb, Lilium polyphyllum D. Don ex Royle have been reported from Chamba, Himachal Pradesh, India for the first time. Karyotypic, palynological, and spermological studies under the light microscope (LM) and scanning electron microscope (SEM) have been carried out on specimens from these populations with an aim to add more knowledge to the current database of the species. Presently studied individuals depicted a diploid (2x) chromosome count of 2n = 24, which is in confirmation with the previous reports from Kashmir Himalaya. Mitotic study showed a karyotype formula of 2n = 2x = 2m + 2sm + 4st + 16t, that is, 2 metacentric +2 submetacentric +4 acrocentric +16 telocentric chromosomes. LM and SEM observations revealed that the pollen grains are monosulcate, heteropolar, elliptical in polar view, and oblate with reticulate ornamentation. Based on the criteria of number, shape, and arrangement of columellae that form the muri, pollen grains in L. polyphyllum correspond to Martagon type. This is the first SEM based study on pollen grains and seeds of endangered Himalayan white lily. Based on the present ecological data collected we confirm the status of L. polyphyllum to be critically endangered in the study area.

中文翻译：

来自喜马拉雅西北部高海拔保护区的新发现的极度濒危药用植物群的核形态学和 SEM 研究 - Lilium polyphyllum D. Don ex Royle

在这项研究中，首次在印度喜马偕尔邦的昌巴报告了一种极度濒危的喜马拉雅草本植物Lilium polyphyllum D. Don ex Royle 的新种群。在光学显微镜 (LM) 和扫描电子显微镜 (SEM) 下对这些种群的标本进行了核型、孢粉学和精子学研究，目的是为当前的物种数据库增加更多知识。目前研究的个体描述了2 n = 24的二倍体 (2 x ) 染色体计数，这与克什米尔喜马拉雅山先前的报告一致。有丝分裂研究显示核型公式为 2 n = 2 x = 2m + 2sm + 4st + 16t，即2个中着丝粒+2个亚中着丝粒+4个近着丝粒+16条端着丝粒染色体。LM和SEM观察表明花粉粒为单槽、异极、极视椭圆形、扁圆状、网纹纹饰。根据形成 muri 的柱状体的数量、形状和排列标准，L. polyphyllum 中的花粉粒对应于 Martagon 类型。这是对濒临灭绝的喜马拉雅白百合花粉粒和种子的第一次基于扫描电镜的研究。根据目前收集到的生态数据，我们确认多叶莲在研究区处于极度濒危状态。

更新日期：2021-04-20

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