Zinc and nitrogen synergistic act on root-to-shoot translocation and preferential distribution in rice,Journal of Advanced Research

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Zinc and nitrogen synergistic act on root-to-shoot translocation and preferential distribution in rice
Journal of Advanced Research ( IF 11.4 ) Pub Date : 2021-04-20 , DOI: 10.1016/j.jare.2021.04.005
Chenchen Ji ₁ , Junli Li ₁ , Cuncang Jiang ₁ , Lin Zhang ₁ , Lei Shi _{1,

2} , Fangsen Xu _{1,

2} , Hongmei Cai ₁

Affiliation

Introduction

Multiple studies have shown strong relationships between different nutrients in plants, and the important role of N in Zn acquisition and translocation has been recognized.

Objectives

The aim of this study was to estimate the effect of Zn on N uptake, translocation, and distribution in rice as well as the corresponding molecular mechanisms. We also aimed to evaluate the impact of N on the Zn content in rice grains which is closely related to the Zn nutrition in humans with rice-based diets.

Methods

We conducted both field trials and hydroponic cultures of two rice cultivars to analyze the growth and yield, the uptake, translocation, and distribution of N and Zn, as well as the expression of N transport and assimilation genes, and the Zn transporter genes under different combined applications of N and Zn.

Results

Zn supply promoted the root-to-shoot translocation (12–70% increasing) and distribution of N into the leaves (19–49% increasing) and brown rice (6–9% increasing) and increased the rice biomass (by 14–35%) and yield (by 13–63%). Zn supply induced the expression of OsNRTs and OsAMTs in both roots and shoots, but repressed the expression of OsNiR2, OsGS1;2, and OsFd-GOGAT in roots, whereas it activated the expression of OsNiR2, OsGS1;1, OsGS2, and OsFd-GOGAT in the shoots. Moreover, the enzyme activities of nitrite reductase, nitrate reductase, and glutamine synthetase increased and the free NO₃^– concentration decreased, but the soluble protein concentration increased significantly in the shoots after Zn supply. Synergistically, N significantly facilitated the root-to-shoot translocation (1.68–11.66 fold) and distribution of Zn into the leaves (1.68–6.37 fold) and brown rice (7–12% increasing) and upregulated the expression levels of Zn transporter genes in both the roots and shoots.

Conclusions

We propose a working model of the cross-talk between Zn and N in rice plants, which will aid in the appropriate combined application of Zn and N fertilizers in the field to improve both N utilization in plants and Zn nutrition in humans with rice-based diets.

中文翻译：

锌氮协同作用对水稻根茎易位和优先分布的影响

介绍

多项研究表明植物中不同养分之间存在密切的关系，N 在 Zn 获取和转运中的重要作用已得到认可。

目标

本研究的目的是估计锌对水稻氮吸收、转运和分布的影响以及相应的分子机制。我们还旨在评估 N 对大米中锌含量的影响，这与以大米为主的饮食中人类的锌营养密切相关。

方法

我们对两个水稻品种进行了田间试验和水培，分析了不同水稻品种的生长和产量、氮和锌的吸收、转运和分布，以及氮转运和同化基因、锌转运蛋白基因的表达情况。氮和锌的联合应用。

结果

锌的供应促进了根到茎的转运（增加 12-70%）和 N 向叶片（增加 19-49%）和糙米（增加 6-9%）的分配，并增加了水稻生物量（增加了 14- 35%）和产量（13-63%）。锌供应诱导根和地上部OsNRTs和OsAMTs的表达，但抑制根中OsNiR2、OsGS1;2和OsFd-GOGAT的表达，而激活OsNiR 2 、OsGS1;1、OsGS2和OsFd的表达- 戈加特在芽中。此外，亚硝酸还原酶、硝酸还原酶和谷氨酰胺合成酶的酶活性增加，游离NO ₃^-施锌后芽中可溶性蛋白浓度降低，但可溶性蛋白浓度显着增加。协同作用，N 显着促进了根到茎的易位（1.68-11.66 倍）和锌在叶片（1.68-6.37 倍）和糙米（增加 7-12%）中的分布，并上调了锌转运蛋白基因的表达水平在根和芽中。