Iron is a crucial trace element and essential for many cellular processes; however, excessive iron accumulation can induce oxidative stress and cell damage. Neurodegenerative disorders, such as Alzheimer’s disease and Parkinson’s disease, have been associated with altered iron homoeostasis causing altered iron distribution and accumulation in brain tissue. This study aims to investigate the protective effect of 1-(N-acetyl-6-aminohexyl)-3-hydroxy-2-methylpyridin-4-one (CM1) in combination with green tea extract (GTE) on iron-induced oxidative stress in neuroblastoma (SH-SY5Y) cells. Cells were cultured in medium with or without ferric chloride loading. Their viability and mitochondrial activity were assessed using MTT and JC-1 staining methods. Levels of the cellular labile iron pool (LIP), reactive oxygen species (ROS), and lipid-peroxidation products were determined using calcein acetoxymethyl ester, 2′,7′-dichlorohydrofluorescein diacetate, and TBARS-based assays, respectively. The viability of iron-loaded cells was found to be significantly increased after treatment with CM1 (10 µM) for 24 h. CM1 co-treatment with GTE resulted in a greater protective effect than their monotherapy. Combination of CM1 and GTE also reduced mitochondrial disruption and LIP content and ROS and TBARS production. In conclusion, the combination of CM1 and GTE exhibits protection against iron-induced oxidative stress in neuroblastoma cells.

中文翻译：

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1-(N-乙酰基-6-氨基己基)-3-羟基-2-甲基吡啶-4-酮和绿茶提取物组合可保护神经母细胞瘤 (SH-SY5Y) 细胞中铁诱导的氧化损伤

铁是一种重要的微量元素，对于许多细胞过程至关重要。然而，过多的铁积累会引起氧化应激和细胞损伤。神经退行性疾病，例如阿尔茨海默病和帕金森病，与铁稳态改变有关，导致铁在脑组织中的分布和积累改变。本研究旨在探讨 1-( N-乙酰基-6-氨基己基)-3-羟基-2-甲基吡啶-4-酮 (CM1) 联合绿茶提取物 (GTE) 对铁诱导的氧化应激的保护作用在神经母细胞瘤（SH-SY5Y）细胞中。细胞在负载或不负载氯化铁的培养基中培养。使用 MTT 和 JC-1 染色方法评估它们的活力和线粒体活性。分别使用钙黄绿素乙酰氧基甲酯、2',7'-二氯氢荧光素二乙酸酯和基于 TBARS 的测定法测定细胞不稳定铁池 (LIP)、活性氧 (ROS) 和脂质过氧化产物的水平。发现用 CM1 (10 µM ) 处理 24 小时后，铁负载细胞的活力显着增加 。CM1 与 GTE 联合治疗比单独治疗产生了更大的保护作用。CM1 和 GTE 的组合还减少了线粒体破坏和 LIP 含量以及 ROS 和 TBARS 的产生。总之，CM1 和 GTE 的组合对神经母细胞瘤细胞中铁诱导的氧化应激具有保护作用。