ABSTRACT

Several algal species were found in the samples collected from a wastewater treatment plant located in Uttarakhand, India. Several media and their modifications were employed to understand the role of these elements and compounds in the species composition and proliferation. Euglena, Chlamydomonas-reinhardtii, Chlorella-sorokiniana, Chlorococcum, several diatoms and cyanobacteria were found in the wastewater samples. In stagnant and flowing municipal wastewater, Euglena, Chlorococcum, cyanobacteria and Volvox were predominant. In a bold basal medium, Scenedesmus and several diatom species were observed along with Chlorococcum. In the presence of acetate Selenastrum species were abundant. Metagenomic analysis of wastewater collected during winter revealed that Tetradesmus wisconsinensis (21.23%) and Actinastrum hantzchii (11.48%) were the dominant species. Some cyanobacterial species accumulated near the high-intensity light region. Hence, subsequent phototaxis experiments were performed. Under the white light, most species showed positive phototaxis. However, under the blue and red light, all the algal species present in the sample showed negative phototaxis. Under the green light, Chlorococcum and Chlamydomonas showed positive phototaxis and Euglena showed negative phototaxis. However, under the pink light, neither negative nor positive phototaxis was observed. Hence, the obtained results provided new insights into the effects of the studied parameters on the species’ diversity and proliferation.

摘要

从位于印度北阿坎德邦的污水处理厂收集的样本中发现了几种藻类。几种媒体及其修改被用来了解这些元素和化合物在物种组成和增殖中的作用。在废水样品中发现了眼虫、莱茵衣藻、小球藻、绿球菌、几种硅藻和蓝藻。在停滞和流动的城市废水中，眼虫、绿球菌、蓝藻和沃尔沃克斯占主导地位。在大胆的基础媒介中，Scenedesmus与绿球菌一起观察到几种硅藻。在醋酸盐的存在下，Selenastrum种类丰富。对冬季收集的废水进行宏基因组分析表明，威斯康星州的 Tetradesmus wisconsinensis (21.23%) 和Actinastrum hantzchii (11.48%) 是优势种。一些蓝藻物种聚集在高强度光照区域附近。因此，进行了随后的趋光性实验。在白光下，大多数物种表现出正趋光性。然而，在蓝光和红光下，样品中存在的所有藻类都表现出负趋光性。在绿灯亮，绿球和衣呈正趋光性，眼虫呈负趋光性。然而，在粉红色光下，既没有观察到负趋光性，也没有观察到正趋光性。因此，获得的结果为研究参数对物种多样性和增殖的影响提供了新的见解。