Abstract

The response of the microbial community (microbial biomass carbon (C_mic), basal respiration (BR), and functional diversity (FD)) of agrosoddy-podzolic soil (Albic Glossic Retisols (Loamic, Aric Cutanic, Ochric)) to pollution by heavy metals (HMs: Cu 660, Zn 1100, Pb 650 mg/kg) and carbon-containing preparations (5% of biochar and 0.25% of lignohumate) was studied in model experiment (30 days). Soils with different organic carbon contents (C_org 3.86 and 1.30%) were sampled at two sites (Chashnikovo, Moscow oblast). We determined C_mic by the substrate-induced respiration method and FD by multisubstrate testing (47 substrates). It was found that HMs application reduced C_mic on average by 49–57%, BR by 23–52%, and FD by 45%, but, on the contrary, increased the microbial metabolic quotient (qCO₂ = BR/C_mic) by 9–46%. The changes of these properties were most significant in the soil with low C_org content (1.30%). Carbon-containing preparations did not contribute to variations in C_mic, BR, and qCO₂ in both soils with HMs, but increased their FD. It is concluded that the studied microbiological parameters may be used as indicators for optimal assessment of soil quality: FD and C_mic are the more sensitive to HMs than BR and qCO₂.

中文翻译：

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腐殖质含量不同的Agrosoddy-Podzolic土壤中重金属和含碳制剂的微生物指标

摘要

重油污染土壤-坡地土壤（Albic Glossic Retisols（Loamic，Aric Cutanic，Ochric））的微生物群落（微生物生物量碳（C _mic），基础呼吸（BR）和功能多样性（FD））对重度污染的响应在模型实验（30天）中研究了金属（HMs：Cu 660，Zn 1100，Pb 650 mg / kg）和含碳制剂（5％的生物炭和0.25％的木质素酸盐）。在两个地点（Chashnikovo，莫斯科州）对具有不同有机碳含量（C _org 3.86和1.30％）的土壤进行了采样。我们通过底物诱导呼吸法确定了C _mic，通过多底物测试（47个底物）确定了FD。发现HMs的应用降低了C _mic平均而言，微生物代谢商（q CO ₂ = BR / C _mic）增加了9–46％，平均增加了49–57％，BR增加了23–52％，FD增加了45 ％。这些特性的变化在低C _org含量（1.30％）的土壤中最为明显。含碳制剂对两种含HM的土壤的C _mic，BR和q CO _2的变化均无贡献，但增加了FD。结论是所研究的微生物学参数可以作为土壤质量最佳评估的指标：FD和C _mic比HM和BR和q CO ₂对HMs更敏感。