ABSTRACT

The cytochrome P450 (CYP) is a large and complex eukaryotic gene superfamily with enzymatic activities involved in several physiological and regulatory processes. As an objective, an in-silico genome-wide DNA methylation (5mC) analysis was performed in rice (Oryza sativa cv. Zhonghua11), and the epigenetic role of CYPs in two abiotic stresses was observed. Being a stable representative mark, DNA-methylation alters the gene expression under stressful environmental conditions. Rice plants under salinity and drought stresses were analyzed through MeDIP-chip hybridization, and 14 unique genes of the CYP family were identified in the rice genome with varying degrees of methylation. The gene structure, promoter sequences, and phylogenetic analysis were performed. Furthermore, the responses of CYPs to various abiotic stresses, including salinity, drought, and cold were revealed. Similarly, the expression profile of potential CYPs was also investigated under various phytohormone stresses, which revealed the potential involvement of CYPs to hormone regulations. Overall, the current study provides evidence for CYP’s stress regulation and fundamental for further characterization and understanding their epigenetic roles in gene expression regulation and environmental stress regulation in higher plants.

摘要

细胞色素 P450 (CYP) 是一个大型而复杂的真核基因超家族，其酶活性参与多种生理和调节过程。作为一个目标，在水稻 ( Oryza sativa ) 中进行了计算机全基因组 DNA 甲基化 (5mC) 分析简历。中华11），并观察了CYP在两种非生物胁迫中的表观遗传作用。作为一个稳定的代表性标记，DNA 甲基化会在压力环境条件下改变基因表达。通过MeDIP芯片杂交分析了盐分和干旱胁迫下的水稻植株，在水稻基因组中鉴定出14个具有不同甲基化程度的CYP家族独特基因。进行了基因结构、启动子序列和系统发育分析。此外，还揭示了 CYPs 对各种非生物胁迫的反应，包括盐度、干旱和寒冷。同样，还在各种植物激素胁迫下研究了潜在 CYP 的表达谱，这揭示了 CYP 可能参与激素调节。全面的，