ABSTRACT

Introduction

The continuous technical improvement in sensitivity and specificity placed mass spectrometry as an alternative method for analyzing clinical samples. In parallel to the rapid development of discovery proteomics, targeted acquisition has been implemented as a complementary option for measuring a small set of proteins with high sensitivity and robustness in a large sample cohort. The combination of trapped ion mobility with a rapid time-of-flight (TOF) mass spectrometer improves the sensitivity even further and triggers the development of prm-PASEF.

Areas covered

This article discusses the development of prm-PASEF and its advantages over the existing targeted and discovery methods for analyzing clinical samples. We are also highlighting the different requirements for the use of prm-PASEF on clinical samples.

Expert opinion

prm-PASEF takes advantage of a dual ion-mobility trap enabling highly multiplexed targeted acquisition. It allows the implementation of a short chromatographic separation setup without sacrificing the number of targeted peptides. Analyzing clinical samples by prm-PASEF holds the promise to significantly improve throughput while maintaining sensitivity to detect the selected target proteins.

中文翻译：

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prm-PASEF质谱的临床潜力

摘要

介绍

灵敏度和特异性的持续技术改进使质谱法成为分析临床样品的替代方法。在发现蛋白质组学的快速发展的同时，靶向采集已被实施作为一种补充选项，用于在大样本队列中测量具有高灵敏度和稳健性的一小组蛋白质。俘获离子迁移率与快速飞行时间 (TOF) 质谱仪的结合进一步提高了灵敏度并引发了 prm-PASEF 的开发。

涵盖的领域

本文讨论了 prm-PASEF 的发展及其相对于现有用于分析临床样本的靶向和发现方法的优势。我们还强调了在临床样本上使用 prm-PASEF 的不同要求。

专家意见

prm-PASEF 利用双离子迁移率阱实现高度多路复用靶向采集。它允许在不牺牲目标肽数量的情况下实施较短的色谱分离设置。通过 prm-PASEF 分析临床样本有望显着提高通量，同时保持检测所选目标蛋白的灵敏度。