The development of plant organs depends on cell division, elongation, structural and chemical changes, and reorganization of cell wall components. As phenotype manipulators, galling insects can manipulate the structure and metabolism of host tissues to build the gall. The gall formation depends on the rearrangement of cell wall components to allow cell growth and elongation, key step for the knowledge regarding gall development, and shape acquisition. Herein, we used an immunocytochemical approach to investigate the chemical composition of the cell wall during the development of galls induced by Bystracoccus mataybae (Eriococcidae) on leaflets of Matayba guianensis (Sapindaceae). Different developmental stages of non-galled leaflets (n = 10) and of leaflet galls (n = 10) were collected from the Cerrado (Brazilian savanna) for anatomical and immunocytochemical analysis. We found that the epitopes of (1 → 4) β-D-galactans and (1 → 5) α-L-arabinans were evident in the tissues of the young and senescent galls. These epitopes seem to be associated with the mechanical stability maintenance and increased gall porosity. As well, the degree of methyl-esterification of pectins changed from the young to the senescent galls and revealed the conservation of juvenile cell and tissue features even in the senescent galls. The extensins detected in senescent galls seem to support their rigidity and structural reinforcement of these bodies. Our results showed a disruption in the pattern of deposition of leaflet cell wall for the construction of M. guianensis galls, with pectin and protein modulation associated with the change of the developmental gall stages.

植物器官的发育取决于细胞分裂，伸长，结构和化学变化以及细胞壁成分的重组。作为表型操纵子，ing虫可以操纵宿主组织的结构和新陈代谢来建立胆汁。胆的形成取决于细胞壁成分的重排以允许细胞生长和伸长，关于胆发展的关键步骤以及形状的获得。在这里，我们使用免疫组织化学方法来研究诱导瘿发展过程中的细胞壁的化学成分Bystracoccus mataybae（Eriococcidae）上的传单Matayba花草（无患子）。无盖小叶的不同发育阶段（n 取自塞拉多（巴西大草原）的小 胆囊（= 10）和小胆囊（n = 10），以进行解剖和免疫细胞化学分析。我们发现（1→4）β-D-半乳糖和（1→5）α-L-阿拉伯糖的抗原表位在年轻和衰老的胆囊组织中很明显。这些表位似乎与维持机械稳定性和增加的孔隙率有关。同样，果胶的甲基酯化程度也从年轻的胆囊变为衰老的胆囊，并且揭示了即使在衰老的胆囊中，青少年细胞和组织特征的保守性。在衰老的胆汁中检测到的延伸蛋白似乎支持它们的刚性和这些身体的结构增强。我们的研究结果表明，在构建小叶的过程中，小叶细胞壁的沉积模式受到了破坏。M. guianensis胆汁，果胶和蛋白质调节与胆汁发育阶段的变化有关。