Plasma electrolytic oxidation (PEO) can be used to obtain oxide coatings able to improve the biocompatibility, wear and corrosion properties of metal implants, due to complex morphology and the formation of oxides with the possibility of incorporating bioactive elements. This study aims at producing coatings on Ti-6Al-4V alloys with potential application for bone tissue repairs, evaluating cytocompatibility behavior and the potential for osteogenic differentiation, by using mesenchymal stem cells. Coatings have been produced on Ti6Al4V alloys by PEO with a calcium and phosphate-based electrolyte, by applying the potentials of 200 V (Ti-PEO_200V) and 300 V (Ti-PEO_300V), with a pulsed electrical regime. The SEM/FEG characterization shown that the samples obtained are typically porous, have different thicknesses and roughness, which were found to be dependent on the maximum applied voltage. The bioactive elements (Ca and P) were incorporated into the coatings and their concentrations grew with the maximum applied voltage, as indicated by RBS analysis. In addition, the same analysis indicated that the coatings were mainly composed of Ti oxides. XRD patterns confirmed that the coatings consist mainly of titanium oxides in the rutile and anatase phases. The tribological properties were evaluated by a ball-on-plate tribometer. Both samples show very similar tribological behavior. The wear resistance of Ti-PEO_300V was more impaired by the microcracks present in its morphology and its great surface roughness than the Ti-PEO_200V sample. Biological properties were assessed by studying the cytocompatibility with MSC cells. The coating obtained for the Ti-PEO_200V sample showed a great cytocompatibility level and initial adhesion, as well as an innate ability for osteo-differentiation of MSCs, displaying considerable potential for usage as bone-repair applications.

等离子体电解氧化（PEO）可用于获得能够改善金属植入物的生物相容性，磨损和腐蚀性能的氧化物涂层，这是由于其复杂的形态和氧化物的形成以及可能掺入生物活性元素的缘故。这项研究旨在通过使用间充质干细胞在Ti-6Al-4V合金上生产具有潜在的骨组织修复涂层，评估细胞相容性行为和成骨分化潜能的涂层。通过施加200 V（Ti-PEO _200V）和300 V（Ti-PEO _300V）的电势，PEO用钙和磷酸盐基电解质在Ti6Al4V合金上生产了涂层），并采用脉冲电方式。SEM / FEG表征表明，所获得的样品通常是多孔的，具有不同的厚度和粗糙度，这取决于最大施加电压。如RBS分析所示，将生物活性元素（Ca和P）掺入涂层中，并且它们的浓度随施加的最大电压而增加。另外，相同的分析表明涂层主要由氧化钛组成。XRD图谱证实了该涂层主要由金红石相和锐钛矿相的氧化钛组成。摩擦学性能通过板球式摩擦计评估。两种样品均显示出非常相似的摩擦学行为。Ti-PEO _300V的耐磨性与Ti-PEO _200V样品相比，它的形貌和较大的表面粗糙度对微裂纹的损害更大。通过研究与MSC细胞的细胞相容性来评估生物学特性。从Ti-PEO _200V样品获得的涂层显示出很高的细胞相容性水平和初始粘附力，以及与生俱来的MSC骨分化能力，显示出可用于骨修复应用的巨大潜力。