Abstract

Purpose

Radiation-induced skin wounds/dermatitis can occur due to therapeutic, occupational, or accidental exposure to ionizing radiation. This study investigated the therapeutic efficacy of standardized Ayurvedic formulations [Jatyadi ghrita (JG) and Jatyadi taila (JT)] against ⁶⁰Co-γ-radiation-induced acute skin wounds in rats.

Material and methods

Animal’s [Sprague-Dawley rats (200 ± 20 g)] flanked skin was locally exposed to 45 Gy radiation (R45Gy) in Cobalt-60-teletherapy unit (Bhabhatron) to generate radiation wounds. JG and JT were applied topically twice daily on wounds from day 14 onwards after appearance of moist desquamation and wound healing efficacy was observed for a period of 42 days.

Results

R45Gy induced significant time dependent changes in rat’s skin with erythema on day 7 followed by dry and moist desquamation. JG and JT application significantly (p < .001) reduced skin damage score, wound area (92% and 97% respectively on day 42), and bacterial load, when compared with R45Gy and showed better efficacy than sucralfate and betamethasone (positive controls). Formulations significantly reduced lipid peroxidation and enhanced antioxidant defenses, reduced inflammatory infiltrates and collagen fibers deposition as evident by decreased myeloperoxidase and hydroxyproline levels, and also reduced transforming growth factor-beta 1 (TGF-β1) expression. Further, histology revealed reduced epidermal hyperplasia and dermal thinning with improved densities of hair follicles. Formulations were found to be nontoxic on 28 days application.

Conclusions

The results demonstrated that JG and JT accelerated wound healing in irradiated skin tissue by faster re-epithelialization; reducing inflammation, collagen fibers deposition, and TGF-β1 expression, indicated their potential human application in countering radiation wounds.

中文翻译：

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局部应用 Jatyadi Ghrita 和 Jatyadi Taila 可加速 Sprague-Dawley 大鼠的伤口愈合：伽马辐射诱导的皮肤伤口模型的研究

 抽象的

 目的

辐射引起的皮肤伤口/皮炎可能是由于治疗、职业或意外暴露于电离辐射而引起的。本研究调查了标准化阿育吠陀制剂 [Jatyadi ghrita (JG) 和 Jatyadi taila (JT)] 对⁶⁰ Co-γ 辐射引起的大鼠急性皮肤伤口的治疗效果。

 材料与方法

将动物[Sprague-Dawley大鼠(200±20g)]侧翼皮肤在Cobalt-60远程治疗装置(Bhabhatron)中局部暴露于45Gy辐射(R45Gy)以产生辐射伤口。从出现湿性脱屑的第14天起，将JG和JT每天两次局部涂抹在伤口上，并观察伤口愈合效果，为期42天。

 结果

R45Gy 诱导大鼠皮肤发生显着的时间依赖性变化，第 7 天出现红斑，随后出现干燥和湿润脱皮。与 R45Gy 相比，JG 和 JT 应用显着 ( p < .001) 降低皮肤损伤评分、伤口面积（第 42 天分别为 92% 和 97%）和细菌载量，并且比硫糖铝和倍他米松（阳性对照）显示出更好的功效）。制剂显着减少脂质过氧化并增强抗氧化防御，减少炎症浸润和胶原纤维沉积（通过降低髓过氧化物酶和羟脯氨酸水平即可证明），并且还减少转化生长因子-β1（TGF-β1）表达。此外，组织学显示表皮增生减少，真皮变薄，毛囊密度提高。使用 28 天后发现制剂无毒。

 结论

结果表明，JG 和 JT 通过更快的上皮再生加速了受辐射皮肤组织的伤口愈合；减少炎症、胶原纤维沉积和 TGF-β1 表达，表明它们在人类对抗辐射伤口方面的潜在应用。