A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles,Nanoscale Horizons

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A plasmon-based nanoruler to probe the mechanical properties of synthetic and biogenic nanosized lipid vesicles
Nanoscale Horizons ( IF 8.0 ) Pub Date : 2021-4-13 , DOI: 10.1039/d1nh00012h
Lucrezia Caselli ₁ , Andrea Ridolfi , Jacopo Cardellini , Lewis Sharpnack , Lucia Paolini , Marco Brucale , Francesco Valle , Costanza Montis , Paolo Bergese , Debora Berti

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Nanosized lipid vesicles are ubiquitous in living systems (e.g. cellular compartments or extracellular vesicles, EVs) and in formulations for nanomedicine (e.g. liposomes for RNA vaccine formulations). The mechanical properties of such vesicles are crucial in several physicochemical and biological processes, ranging from cellular uptake to stability in aerosols. However, their accurate determination remains challenging and requires sophisticated instruments and data analysis. Here we report the first evidence that the surface plasmon resonance (SPR) of citrated gold nanoparticles (AuNPs) adsorbed on synthetic vesicles is finely sensitive to the vesicles’ mechanical properties. We then leverage this finding to show that the SPR tracking provides quantitative access to the stiffness of vesicles of synthetic and natural origin, such as EVs. The demonstration of this plasmon-based “stiffness nanoruler” paves the way for developing a facile, cost-effective and high-throughput method to assay the mechanical properties of dispersions of vesicles of nanometric size and unknown composition at a collective level.

中文翻译：

一种基于等离子体的纳米尺，用于探测合成和生物纳米脂质囊泡的机械性能

纳米脂质囊泡在生命系统（例如细胞隔室或细胞外囊泡，EVs）和纳米药物制剂（例如用于 RNA 疫苗制剂的脂质体）。这种囊泡的机械特性在几个物理化学和生物过程中至关重要，从细胞摄取到气溶胶中的稳定性。然而，它们的准确测定仍然具有挑战性，需要复杂的仪器和数据分析。在这里，我们报告了第一个证据，即吸附在合成囊泡上的柠檬酸金纳米粒子 (AuNPs) 的表面等离子体共振 (SPR) 对囊泡的机械性能非常敏感。然后，我们利用这一发现来表明 SPR 跟踪提供了对合成和天然来源的囊泡刚度的定量访问，例如 EV。这种基于等离子体的“刚度纳米尺”的演示为开发一种简便的方法铺平了道路，

更新日期：2021-04-19

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