Transcription factors regulate gene expression depending on environmental and extracellular stimuli and signals from other cells. WRKY, MYB, bHLH and bZIP transcription factors (TFs) are one of the most important TF families in plants. Therefore, WRKY, MYB, bHLH and bZIP TFs in potato (Solanum tuberosum, St) genome under heat, salinity and drought stresses were investigated in terms of expression, co-expression and differentially expressed genes (DEGs). A total of 26,172 genes were collectively analysed in the study and four genes were identified as common DEGs, highly expressed under each abiotic stress. The salt stress was found to induce StbHLH and StMYB TFs whereas the heat stress activated StWRKY and StbZIP TFs. Moreover, the top three highly expressed genes were identified for each TF family under each stress condition. Since some of the genes were unannotated, their functional predictions including unannotated common DEGs were made based on orthologue gene comparisons in Arabidopsis. TFs involved in gene regulation along with four common DEGs were identified in co-expression analyses. As a result, 12 bHLH, 5 bZIP, 9 MYB and 14 WRKY TFs were detected in co-expression networks of the four common DEGs. Regarding DEGs, PGSC0003DMT400040149 was generally found to be involved in sulphur metabolism. PGSC0003DMT400016360 and PGSC0003DMT400007351 gene networks were found to contain more than 400 overlapping genes, suggesting that they may have roles in similar pathways in response to abiotic stress conditions in potato. Lastly, we found that MYB-bHLH cross talking may occur in response to abiotic stresses in potato. The findings are particularly important to understand roles of TFs under the mentioned stresses in potato.

转录因子根据环境和细胞外刺激以及来自其他细胞的信号调节基因表达。WRKY，MYB，bHLH和bZIP转录因子（TFs）是植物中最重要的TF家族之一。因此，马铃薯（马铃薯）中的WRKY，MYB，bHLH和bZIP TFs，St）基因组在热，盐和干旱胁迫下的表达，共表达和差异表达基因（DEG）进行了研究。在这项研究中，总共分析了26,172个基因，并确定了四个基因是常见的DEG，它们在每种非生物胁迫下均高表达。发现盐胁迫诱导StbHLH和StMYB TF，而热胁迫激活StWRKY和StbZIP TF。此外，在每个应激条件下，每个TF家族鉴定出了前三个高表达基因。由于某些基因是未注释的，因此根据拟南芥中直向同源基因的比较来做出其功能预测，包括未注释的常见DEG。。在共表达分析中鉴定了参与基因调控的TF和四个常见的DEG。结果，在四个常见DEG的共表达网络中检测到了12个bHLH，5个bZIP，9个MYB和14个WRKY TF。关于DEG，通常发现PGSC0003DMT400040149参与硫代谢。发现PGSC0003DMT400016360和PGSC0003DMT400007351基因网络包含400多个重叠基因，表明它们可能在响应马铃薯非生物胁迫条件的相似途径中发挥作用。最后，我们发现MYB - bHLH土豆中的非生物胁迫可能会引起串扰。这些发现对于理解TF在马铃薯中提到的胁迫下的作用特别重要。