The purpose of this study was to detect the immunomodulatory activity of Indigofera coerulea on the cytokine's expression in the THP-1 human cell line. THP-1 cell lines were differentiated into real macrophages. Cell's viability was assessed by MTT assay. The qRT-PCR assay was used to determine the effects of the I. coerulea extract on mRNA expression levels in THP-1 cells. The effects of extracts on proteins production were analyzed by Western blot assay and protein array. The ELISA was used to determine the effect of the plant extract on cytokine expression. Finally, apoptosis, phagocytosis, and cell migration assays were performed to investigate the effects of the extract on macrophage functions. The obtained results illustrated that I. coerulea extract possesses anti-oxidant and anti-inflammatory activities. In addition, current study reported that I. coerulea extract significantly reduced the proliferation of THP-1 cells at all-time points. Moreover, I. coerulea extract showed significant immunomodulatory activity compared with control macrophages by influencing tumor necrosis factor-α (TNF-α), IL-1β, IL-6, CCL22, CXCL10/IP-10, CXCL8/IL-8, ERK5, BAX, BcL2, Cyclin D1, ERK1, P-IκB-α, P-NF-κB, and P-p38 proteins and the signaling pathways of NF-κB, p38 MAPK, ERK1/2, and IL-6/JAK/STAT3. In conclusion, this study is the first to underline the anti-proliferative, anti-inflammatory, anti-phagocytic, anti-apoptotic, and anti-migratory properties of the studied plant extract in human monocytic THP-1 cells.

这项研究的目的是检测蓝靛蓝对THP-1人细胞系中细胞因子表达的免疫调节活性。THP-1细胞系分化为真正的巨噬细胞。通过MTT测定法评估细胞的生存力。使用qRT-PCR测定法测定蓝藻提取物对THP-1细胞中mRNA表达水平的影响。通过蛋白质印迹法和蛋白质阵列分析提取物对蛋白质生产的影响。ELISA用于确定植物提取物对细胞因子表达的影响。最后，进行细胞凋亡，吞噬作用和细胞迁移分析，以研究提取物对巨噬细胞功能的影响。获得的结果说明蓝藻提取物具有抗氧化和抗炎活性。此外，目前的研究报道，蓝藻提取物在所有时间点均显着降低了THP-1细胞的增殖。此外，与对照巨噬细胞相比，蓝藻提取物通过影响肿瘤坏死因子-α（TNF-α），IL-1β，IL-6，CCL22，CXCL10 / IP-10，CXCL8 / IL-8，ERK5而显示出显着的免疫调节活性。 ，BAX，BcL2，细胞周期蛋白D1，ERK1，P-IκB-α，P-NF-κB和P-p38蛋白以及NF-κB，p38 MAPK，ERK1 / 2和IL-6 / JAK /的信号通路STAT3。总之，这项研究首次强调了所研究植物提取物在人单核THP-1细胞中的抗增殖，抗炎，抗吞噬，抗凋亡和抗迁移特性。