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In vitro investigation of antioxidant and antihemolytic activities of three Lamiaceae species from Morocco
Beni-Suef University Journal of Basic and Applied Sciences ( IF 2.5 ) Pub Date : 2021-04-17 , DOI: 10.1186/s43088-021-00116-9
Abdelbassat Hmidani , Eimad dine Tariq Bouhlali , Mohammed Ajebli , Tarik Khouya , Mohamed Benlyas , Chakib Alem

Lkhzama (Lavandula officinalis), Mard-doch (Origanum majorana), and Lahbak (Ocimum basilicum) are aromatic and medicinal plants widely used in Moroccan folk medicine as a treatment for numerous diseases including liver diseases, rheumatism, and diabetes. This study was undertaken to examine the antioxidant and antihemolytic activities of the aqueous extracts of these plants. The antioxidant activity was evaluated using three in vitro tests: DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) radical scavenging activity, FRAP (ferric reducing antioxidant power assay), and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging assay. The antihemolytic activity of plant extracts was evaluated against AAPH (2,2′-azobis(2-amidino-propane) dihydrochloride)-induced erythrocyte hemolysis. Our findings showed that all plant extracts displayed significant antioxidant and antihemolytic effects. In fact, among the studied plant extracts, the highest antioxidant power was recorded in Origanum majorana, based on DPPH (IC50 = 12.29 μg/mL), ABTS (226.13 μmol TE/g DW), and FRAP (477.82 μmol TE/g DW) assays. Moreover, the same plant also showed the best membrane protective effect (269.55%). Whereas, Ocimum basilicum exhibited the lowest antioxidant activity using DPPH (IC50 = 42.85 μg/mL), ABTS (IC50 = 226.13 μmol TE/g DW), and FRAP (IC50 = 172.84 μmol TE/g DW) and, thus, the lowest membrane protective effect (182.70%). This result supports the use of these plants in folk medicine for preventing and treating many diseases, especially those related to oxidative stress.

中文翻译:

摩洛哥三种唇形科的抗氧化和抗溶血活性的体外研究

Lkhzama(Lavandula officinalis),Mard-doch(Origanum majorana)和Lahbak(Ocimum basilicum)是芳香族和药用植物,广泛用于摩洛哥民间医学,用于治疗多种疾病,包括肝病,风湿病和糖尿病。进行这项研究以检查这些植物的水提取物的抗氧化和抗溶血活性。使用三种体外测试评估了抗氧化活性:DPPH(2,2-二苯基-1-吡啶基-肼基-水合物)自由基清除活性,FRAP(还原铁抗氧化能力测定)和ABTS(2,2'-azino-双(3-乙基苯并噻唑啉-6-磺酸)自由基清除试验。评价了植物提取物对AAPH(2,2'-偶氮双(2-ami基丙烷)二盐酸盐)诱导的红细胞溶血的抗溶血活性。我们的发现表明,所有植物提取物均显示出显着的抗氧化和抗溶血作用。实际上,在研究的植物提取物中,基于DPPH(IC50 = 12.29μg/ mL),ABTS(226.13μmolTE / g DW)和FRAP(477.82μmolTE / g DW),牛至中的抗氧化能力最高。 )分析。此外,同一株植物也表现出最佳的膜保护效果(269.55%)。相比之下,罗勒叶(Ocimum basilicum)在DPPH(IC50 = 42.85μg/ mL),ABTS(IC50 = 226.13μmolTE / g DW)和FRAP(IC50 = 172.84μmolTE / g DW)时表现出最低的抗氧化活性,因此是最低的膜保护作用(182.70%)。该结果支持这些植物在民间医学中用于预防和治疗许多疾病,尤其是与氧化应激有关的疾病的用途。在所研究的植物提取物中,基于DPPH(IC50 = 12.29μg/ mL),ABTS(226.13μmolTE / g DW)和FRAP(477.82μmolTE / g DW)分析,牛至中的抗氧化能力最高。此外,同一株植物也表现出最佳的膜保护效果(269.55%)。相比之下,罗勒叶(Ocimum basilicum)在DPPH(IC50 = 42.85μg/ mL),ABTS(IC50 = 226.13μmolTE / g DW)和FRAP(IC50 = 172.84μmolTE / g DW)时表现出最低的抗氧化活性,因此是最低的膜保护作用(182.70%)。该结果支持这些植物在民间医学中用于预防和治疗许多疾病,尤其是与氧化应激有关的疾病的用途。在所研究的植物提取物中,基于DPPH(IC50 = 12.29μg/ mL),ABTS(226.13μmolTE / g DW)和FRAP(477.82μmolTE / g DW)分析,牛至中的抗氧化能力最高。此外,同一株植物也表现出最佳的膜保护效果(269.55%)。相比之下,罗勒叶(Ocimum basilicum)在DPPH(IC50 = 42.85μg/ mL),ABTS(IC50 = 226.13μmolTE / g DW)和FRAP(IC50 = 172.84μmolTE / g DW)时表现出最低的抗氧化活性,因此是最低的膜保护作用(182.70%)。该结果支持这些植物在民间医学中用于预防和治疗许多疾病,尤其是与氧化应激有关的疾病的用途。82μmolTE / g DW)分析。此外,同一株植物还表现出最佳的膜保护效果(269.55%)。相比之下,罗勒叶(Ocimum basilicum)在DPPH(IC50 = 42.85μg/ mL),ABTS(IC50 = 226.13μmolTE / g DW)和FRAP(IC50 = 172.84μmolTE / g DW)时表现出最低的抗氧化活性,因此是最低的膜保护作用(182.70%)。该结果支持这些植物在民间医学中用于预防和治疗许多疾病,尤其是与氧化应激有关的疾病的用途。82μmolTE / g DW)分析。此外,同一株植物也表现出最佳的膜保护效果(269.55%)。相比之下,罗勒叶(Ocimum basilicum)在DPPH(IC50 = 42.85μg/ mL),ABTS(IC50 = 226.13μmolTE / g DW)和FRAP(IC50 = 172.84μmolTE / g DW)时表现出最低的抗氧化活性,因此是最低的膜保护作用(182.70%)。该结果支持这些植物在民间医学中用于预防和治疗许多疾病,尤其是与氧化应激有关的疾病的用途。
更新日期:2021-04-18
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