Abstract

The present study was designed to evaluate the effect of factors like hormones, antisperm antibody (ASA), and oxidative stress and its relation with semen quality in crossbred bulls. Ejaculates from two bulls were categorized into good (n = 12) and poor (n = 12) based on initial progressive motility, that is, ≥70% and ≤50%, respectively. The level of hormones like Testosterone (p < 0.05) and PGE₂ (p < 0.01) was significantly higher in good-quality ejaculates compared to poor-quality ejaculates; however, estradiol (p < 0.05), progesterone, oxidative stress, and ASAs were significantly higher (p < 0.01) in poor-quality ejaculates compared to good-quality ejaculates. Therefore, it could be concluded that oxidative stress and hormonal imbalance might have resulted in high number of dead and defective spermatozoa which was ultimately responsible for poor quality semen ejaculates.

摘要

本研究旨在评估激素、抗精子抗体 (ASA) 和氧化应激等因素的影响及其与杂交公牛精液质量的关系。 根据初始前向运动性，即分别≥70% 和≤50%，将两头公牛的精液分为好 ( n  = 12) 和差 ( n = 12)。 与劣质射精相比，优质射精中睾酮 ( p  < 0.05) 和 PGE ₂ ( p < 0.01)等激素水平明显更高；然而，雌二醇 ( p  < 0.05)、孕酮、氧化应激和 ASAs 显着更高 ( p < 0.01) 与优质射精相比，劣质射精。因此，可以得出结论，氧化应激和荷尔蒙失调可能导致大量死亡和有缺陷的精子，最终导致精液质量差。