The present study aimed to analyse and compare the distribution of MICA (rs1051792) and NKG2D/KLRK1 (rs1154831, rs1049174, rs2255336) polymorphisms in 61 Greek and 100 Polish patients with rheumatoid arthritis in relation to the presence of the HLA-DRB1 shared epitope and clinical parameters. Genotyping of selected polymorphism was performed using real-time PCR. HLA-DRB1 shared epitope alleles segregated differently in Greek and Polish patients but in both populations were detected in over 60% of cases. The rs1051792-A variant was more common among SE-positive Polish patients (p = 0.003) while the rs1049174-G allele was more frequently observed in Greeks than in Poles (p < 0.001). Moreover, among Greek patients, the rs1051792-GG homozygotes more frequently presented with anti-CCP antibodies and rheumatoid factor (RF), while carriers of the rs1049174-G variant and rs1154831-CC homozygotes were characterized by lower disease activity scores (p < 0.05 in all cases). These results imply that, in addition to the HLA-DRB1 SE alleles, MICA and NKG2D polymorphisms may also play a role in rheumatoid arthritis.

中文翻译：

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希腊和波兰类风湿关节炎患者的MICA和NKG2D编码基因共有表位和多态性

本研究旨在分析和比较MICA（rs1051792）和NKG2D / KLRK1（rs1154831，rs1049174，rs2255336）多态性在HLA-DRB1共有表位和临床参数。使用实时PCR对选定的多态性进行基因分型。HLA-DRB1共有的抗原决定簇等位基因在希腊和波兰患者中的分离方式不同，但在这两个人群中，超过60％的病例均被检测到。rs1051792-A变异在SE阳性的波兰患者中更为常见（p = 0.003），而在希腊人中比在波兰人中更常见rs1049174-G等位基因（p <0.001）。此外，在希腊患者中，rs1051792-GG纯合子更常出现抗CCP抗体和类风湿因子（RF），而rs1049174-G变体和rs1154831-CC纯合子的携带者具有较低的疾病活动性评分（在所有情况下p <0.05）。这些结果表明，除了HLA-DRB1 SE等位基因外，MICA和NKG2D多态性也可能在类风湿关节炎中起作用。