ABSTRACT

Tendon is a fibrous connective tissue where the fibres as biophysical cues are key regulators to regenerate injured tendon. However, electrospun fibres are often mechanically insufficient for the tendon application. Here, we reported a method by using rough polymer surface as the reinforcement of fibres to promote surface fibre deposition and integration during electrospinning. Results showed that the addition of polymer surface with open-pore topography significantly improved the deposition of fibres. The as-fabricated fibre topography was found to couple with improved surface hydrophilicity, higher crystallinity, and mechanical properties including both the maximum force and extension upon receiving elastic and plastic deformations. Seeding with TCSs, the fibre topography showed good cell compatibility and could be able to support F-actin cytoskeleton expression with the building of tendon cell interconnection. This work could have insights into the design of mechanically enhanced fibre topography for tendon tissue regeneration.

摘要

肌腱是一种纤维结缔组织，其中作为生物物理线索的纤维是再生受损肌腱的关键调节剂。然而，电纺纤维通常在机械上不足以用于肌腱应用。在这里，我们报道了一种通过使用粗糙的聚合物表面作为纤维的增强材料来促进静电纺丝过程中表面纤维沉积和整合的方法。结果表明，添加具有开孔形貌的聚合物表面显着改善了纤维的沉积。发现所制造的纤维形貌与改善的表面亲水性、更高的结晶度和机械性能相结合，包括在接受弹性和塑性变形时的最大力和延伸。用 TCS 播种，纤维形貌显示出良好的细胞相容性，并且能够通过肌腱细胞互连的建立来支持 F-肌动蛋白细胞骨架的表达。这项工作可以深入了解用于肌腱组织再生的机械增强纤维形貌的设计。