Bacterial disinfection and cell assessment post ultraviolet-C LED exposure for wound treatment,Medical & Biological Engineering & Computing

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Bacterial disinfection and cell assessment post ultraviolet-C LED exposure for wound treatment
Medical & Biological Engineering & Computing ( IF 2.6 ) Pub Date : 2021-04-18 , DOI: 10.1007/s11517-021-02360-8
Jahanzeb Sheikh ₁ , Tan Tian Swee ₁ , Syafiqah Saidin _{1,

2} , Azli Bin Yahya ₁ , Sameen Ahmed Malik ₁ , Joyce Sia Sin Yin ₁ , Matthias Tiong Foh Thye ₁

Affiliation

Ultraviolet-C sourced LED (UVC-LED) has been widely used for disinfection purposes due to its germicidal spectrum. In this study, the efficiencies of UVC-LED for Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) disinfections were investigated at three exposure distances (1, 1.5, and 2 cm) and two exposure times (30 and 60 s). The respective bacterial inhibition zones were measured, followed by a morphological analysis under SEM. The viabilities of human skin fibroblast cells were further evaluated under the treatment of UVC-LED with the adoption of aforesaid exposure parameters. The inhibition zones were increased with the increment of exposure distances and times. The highest records of 5.40 ± 0.10 cm P. aeruginosa inhibition and 5.43 ± 0.11 cm S. aureus inhibition were observed at the UVC-LED distance of 2 cm and 60-s exposure. Bacterial physical damage with debris formation and reduction in size were visualized following the UVC-LED exposures. The cell viability percentages were in a range of 75.20–99.00% and 82–100.00% for the 30- and 60-s exposures, respectively. Thus, UVC-LED with 275-nm wavelength is capable in providing bacterial disinfection while maintaining accountable cell viability which is suitable to be adopted in wound treatment.

Graphical abstract

中文翻译：

用于伤口治疗的紫外线-C LED 暴露后的细菌消毒和细胞评估

紫外线-C 光源 LED (UVC-LED) 由于其杀菌光谱而被广泛用于消毒目的。在这项研究中，的效率UVC-LED为铜绿假单胞菌（绿脓杆菌）和金黄色葡萄球菌（金黄色葡萄球菌）消毒在三个曝光距离（1,1.5，和2厘米）和两个曝光时间（30和60进行了研究s)。测量各自的抑菌圈，然后在扫描电镜下进行形态分析。采用上述曝光参数进一步评估了在UVC-LED处理下人皮肤成纤维细胞的活力。抑菌圈随着暴露距离和次数的增加而增加。5.40±0.10厘米的最高记录在 2 厘米和 60 秒的 UVC-LED 距离下观察到铜绿假单胞菌抑制和 5.43 ± 0.11 厘米金黄色葡萄球菌抑制。在 UVC-LED 曝光后，细菌物理损伤与碎片形成和尺寸减小被可视化。对于 30 秒和 60 秒的暴露，细胞活力百分比分别在 75.20-99.00% 和 82-100.00% 的范围内。因此，波长为 275 nm 的 UVC-LED 能够提供细菌消毒，同时保持可靠的细胞活力，适用于伤口治疗。

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更新日期：2021-04-18

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