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Molecular detection and genetic characterization of ovine gammaherpesvirus-2 (OvHV-2) in sheep in the Philippines
Small Ruminant Research ( IF 1.6 ) Pub Date : 2021-04-16 , DOI: 10.1016/j.smallrumres.2021.106383
Innina R. Mananguit , Nest Dale F. Bartolome , Gabriel Alexis S.P. Tubalinal , Claro N. Mingala

Ovine gammaherpesvirus-2 (OvHV-2) causes sheep-associated malignant catarrhal fever (SA-MCF) and is a fatal disease of some ruminants such as cattle, water buffalo and even-toed ungulates (such as deer, other cervids and some species of pigs). OvHV-2, however, was never reported to be present in the Philippines. Thus, this study is conducted. Out of 89 sheep blood samples from 6 farms, 58 (65.17 %) were positive for OvHV-2 using semi-Polymerase Chain Reaction targeting the tegument gene. Phylogenetic analysis suggests that the OvHV-2 sequences from sheep in the Philippines are nearly identical to the OvHV-2 sequences of animals from Brazil and Germany and 98 % identical to sequences from Turkey, India, Egypt, UK, and Mongolia. To date, this is the first report of OvHV-2 in the Philippines and could provide information in the future for the control and prevention of SA-MCF in the country.



中文翻译:

菲律宾绵羊羊γ疱疹病毒2(OvHV-2)的分子检测和遗传特征

绵羊γ疱疹病毒2(OvHV-2)会引起绵羊相关的恶性卡他热(SA-MCF),并且是一些反刍动物的致命疾病,例如牛,水牛和有趾的有蹄类动物(例如鹿,其他鹿和某些猪)。然而,从未有报道称OvHV-2在菲律宾存在。因此,进行了这项研究。在来自6个农场的89个绵羊血液样本中,有58个(65.17%)使用靶向tegument基因的半聚合酶链反应对OvHV-2呈阳性。系统发育分析表明,菲律宾绵羊的OvHV-2序列与巴西和德国动物的OvHV-2序列几乎相同,并且与土耳其,印度,埃及,英国和蒙古的序列相近98%。迄今为止,

更新日期:2021-04-16
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