A halotolerant bacterial strain was isolated from oily-contaminated sites of Persian Gulf, which characterized as Pseudomonas aeruginosa (AHV-KH10) by 16S rRNA gene sequencing. This strain was used for bioremediation of diesel-contaminated sediments. Biosurfactant production was initially screened by using oil displacement test and drop-collapse method, followed by measurement of surface tension (ST) of growth medium. Produced biosurfactant was a rhamnolipid type biosurfactant and lowered the ST to 33.4 mN/m at the given critical micelle concentration (CMC) of 75 mg/L. Addition of 3 CMC rhamnolipid, inoculums size of 15 mL, biodegradation in slurry phase and salinity level of 6% led totally to a diesel biodegradation rate of 70% for initial concentration of 1000 mg/kg after 35 days. The maximum diesel removal occurred at the salinity content of 6% indicating the moderately halo-tolerant characteristics of isolated strain. Evaluation of bacterial growth showed a biomass yield of 0.33 mg _VSS/mg _diesel in selected conditions. The field performance of Pseudomonas aeruginosa AHV-KH10 was proved through the removal of the TPH content in unwashed sediment, which varied from 2390 to 1875 mg/kg within four months.

从波斯湾的油性污染场所分离出一种卤化细菌菌株，其特征为铜绿假单胞菌（AHV-KH10）通过16S rRNA基因测序。该菌株用于柴油污染的沉积物的生物修复。首先通过驱油试验和跌落塌陷法筛选生物表面活性剂的生产，然后测量生长培养基的表面张力（ST）。生产的生物表面活性剂是鼠李糖脂型生物表面活性剂，在给定的临界胶束浓度（CMC）为75 mg / L时，ST降低至33.4 mN / m。添加3种CMC鼠李糖脂，接种物大小为15 mL，淤浆相生物降解和盐度为6％，在35天后初始浓度为1000 mg / kg时，柴油的生物降解率为70％。在含盐量为6％时发生了最大的柴油去除，表明分离菌株具有中等耐盐性。细菌生长评估显示生物量为0.33 mg在选定条件下为_VSS / mg_柴油。铜绿假单胞菌AHV-KH10的田间性能通过去除未冲洗沉积物中TPH的含量得以证明，该含量在四个月内从2390到1875 mg / kg不等。