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Determination of antioxidant activity and phenolic compounds for basic standardization of Turkish propolis
Applied Biological Chemistry ( IF 2.3 ) Pub Date : 2021-04-16 , DOI: 10.1186/s13765-021-00608-3
Aslı Özkök 1 , Merve Keskin 2 , Aslı Elif Tanuğur Samancı 3 , Elif Yorulmaz Önder 3 , Çiğdem Takma 4
Affiliation  

This study aimed to determine the standard amount of antioxidant content and compounds of the propolis for the standardization of propolis. For this purpose, the total flavonoids, total phenolic, CUPRAC antioxidant capacity content and the diversity of phenolic and flavonoid components of these propolis samples were found by HPLC determined at the 23 propolis samples which were collected different regions of Turkey. Beside that, the similarities and differences of these 23 provinces to each other according to their antioxidant capacities were investigated by multidimensional scaling analysis. The total flavonoid content in the propolis samples were determined between 21.28 and 152.56 mg CE/g. The total phenolic content in the propolis samples was found between 34.53 mg and 259.4 mg GAE/g. CUPRAC antioxidant capacity of the propolis samples and antioxidant range was found from 95.35 to 710.43 mg TE/g. Also, 4 flavonoid [Quercetin (min.1.12–max.4.14 mg/g), Galangin (min.0.72–max.40.79 mg/g), Apigenin (min.1.07–max.17.35 mg/g), Pinocembrin (min.1.32–max.39.92 mg/g] and 6 phenolic acid [Caffeic acid (min.1.20–max.7.6 mg/g), p-Coumaric acid (min.1.26–max.4.47 mg/g), trans-Ferulic acid (min.1.28–max.4.92 mg/g), Protocatechuic acid (1.78 mg/g), trans-Cinnamic acid (min.1.05–max.3.83 mg/g), Caffeic Acid Phenethyl Ester (CAPE) (min.1.41–max.30.15 mg/g)] components were detected as mg/g, in different ratios in propolis samples collected from different regions. The feature of this study, so far, is to have the maximum number of samples representing the Turkish propolis, and so is thought to help to national and international propolis standard workings.

中文翻译:

土耳其蜂胶基本标准化的抗氧化活性和酚类化合物的测定

本研究旨在确定蜂胶中抗氧化剂含量和化合物的标准量,用于蜂胶的标准化。为此,通过 HPLC 测定从土耳其不同地区收集的 23 个蜂胶样品,发现这些蜂胶样品的总黄酮类化合物、总酚类物质、CUPRAC 抗氧化能力含量以及酚类和类黄酮成分的多样性。此外,通过多维尺度分析研究了这23个省份在抗氧化能力方面的异同。蜂胶样品中的总类黄酮含量测定为 21.28 至 152.56 mg CE/g。蜂胶样品中的总酚含量在 34.53 mg 和 259.4 mg GAE/g 之间。蜂胶样品的 CUPRAC 抗氧化能力和抗氧化范围为 95.35 至 710.43 mg TE/g。此外,4 种类黄酮 [槲皮素(最小 1.12–最大 4.14 mg/g)、高良姜素(最小 0.72–最大 40.79 mg/g)、芹菜素(最小 1.07–最大 17.35 mg/g)、松木素(最小.1.32–max.39.92 mg/g] 和 6 酚酸 [咖啡酸(min.1.20–max.7.6 mg/g),对香豆酸(min.1.26–max.4.47 mg/g),反式阿魏酸酸(min.1.28–max.4.92 mg/g)、原儿茶酸(1.78 mg/g)、反式肉桂酸(min.1.05–max.3.83 mg/g)、咖啡酸苯乙酯 (CAPE)(min. 1.41–max.30.15 mg/g)] 成分被检测为 mg/g,在从不同地区收集的蜂胶样品中以不同的比例检测。这项研究的特点,到目前为止,是有最大数量的样品代表土耳其蜂胶,
更新日期:2021-04-16
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