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NAC transcription factors SNAC4 and SNAC9 synergistically regulate tomato fruit ripening by affecting expression of genes involved in ethylene and abscisic acid metabolism and signal transduction
Postharvest Biology and Technology ( IF 6.4 ) Pub Date : 2021-04-15 , DOI: 10.1016/j.postharvbio.2021.111555
Sen Yang , Jiaqian Zhou , Christopher B. Watkins , Caie Wu , Yanchun Feng , Xiaoyang Zhao , Zhaohui Xue , Xiaohong Kou

NAC (NAM, ATF1/2 and CUC2) is a transcription factor that can regulate many physiological and biochemical reactions in plants. Two NAC genes, SNAC4 (NM_001279348.2) and SNAC9 (NM_001365397.1), with similar secondary structures have been isolated from tomato (Solanum lycopersicum L.). The pattern of SNAC4/9 co-regulation of tomato ripening with abscisic acid (ABA) and ethylene through yeast two-hybrid and bimolecular fluorescence complementation assays has revealed that SNAC4/9 can interact with genes related to ABA and ethylene (SAPK3, SlPYL9, SlAREB1, SlACS2 and SlACO1) at the protein level. Electrophoretic mobility shift assay and yeast one-hybrid showed that SNAC4, but not SNAC9, acts directly on the promoter regions of SAPK3, SlCYP707A1, SlACS8 and SlACO6 and can activates them. In addition, quantitative real-time PCR analyses of SNAC4/9-silenced fruit obtained by virus-induced gene silencing, and phytohormone-treated fruit, confirmed that the interaction genes are regulated by SNAC4/9 and thereby affect fruit ripening. In summary, we found that SNAC4/9 can regulate fruit ripening by positively acting on key genes in the synthesis and signal transduction of ABA and ethylene. SNAC4/9 cooperates with these phytohormones as part of the tomato fruit ripening regulatory network.



中文翻译:

NAC转录因子SNAC4SNAC9通过影响参与乙烯和脱落酸代谢和信号转导的基因的表达来协同调节番茄果实的成熟

NAC(NAM,ATF1 / 2和CUC2)是一种转录因子,可以调节植物中的许多生理和生化反应。两个NAC基因,SNAC4(NM_001279348.2)和SNAC9(NM_001365397.1),具有类似的二级结构已经从番茄中分离(番茄L.)。SNAC4 / 9通过酵母双杂交和双分子荧光互补分析共同调控脱落酸(ABA)和乙烯成熟的番茄的模式表明,SNAC4 / 9可以与ABA和乙烯相关的基因相互作用(SAPK3,SlPYL9, SlAREB1,SlACS2SlACO1)的蛋白质水平。电泳迁移率迁移分析和酵母一杂交表明,SNAC4,而不是SNAC9,直接作用于SAPK3,SlCYP707A1,SlACS8S1ACO6的启动子区域,并可以激活它们。另外,通过病毒诱导的基因沉默获得的SNAC4 / 9沉默的果实和植物激素处理的果实的定量实时PCR分析证实,相互作用基因受SNAC4 / 9调控,从而影响果实的成熟。总之,我们发现SNAC4 / 9可通过积极作用于ABA和乙烯的合成和信号转导中的关键基因来调节果实成熟。SNAC4 / 9 与这些植物激素合作,作为番茄果实成熟调控网络的一部分。

更新日期:2021-04-16
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