Amyotrophic lateral sclerosis (ALS) is an incurable neurodegenerative disease that primarily affects motor neurons, causing muscle atrophy, bulbar palsy, and pyramidal tract signs. However, the aetiology and pathogenesis of ALS have not been elucidated to date. In this study, a competitive endogenous RNA (ceRNA) network was constructed by analyzing the expression profiles of messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) that were matched by 7 ALS samples and 4 control samples, and then a protein-protein interaction (PPI) network was constructed to identify the genes related to ALS. Gene Ontology (GO) was used to study the potential functions of differentially expressed mRNAs (DEmRNAs) in the ceRNA network. For the ALS and control groups, 247177 potential lncRNA-mRNA ceRNA relationship pairs were screened. Analysis of significant relationship pairs demonstrated that the PPI modules formed by the MALAT1-regulated SYNRG, ITSN2, PICALM, AP3B1, and AAK1 genes may play important roles in the pathogenesis of ALS, and these results may help to characterize the pathogenesis of ALS.

中文翻译：

---

lncRNA-miRNA-mRNA 轴在肌萎缩侧索硬化中的新调控作用：综合生物信息学分析

肌萎缩侧索硬化 (ALS) 是一种无法治愈的神经退行性疾病，主要影响运动神经元，导致肌肉萎缩、延髓麻痹和锥体束征。然而，迄今为止，ALS 的病因和发病机制尚未阐明。在本研究中，通过分析与 7 个 ALS 样本和 4 个对照样本匹配的信使 RNA (mRNA) 和长链非编码 RNA (lncRNA) 的表达谱，构建了竞争性内源 RNA (ceRNA) 网络，然后构建了一个蛋白质-蛋白质构建相互作用（PPI）网络以识别与ALS相关的基因。基因本体论 (GO) 用于研究 ceRNA 网络中差异表达的 mRNA (DEmRNA) 的潜在功能。对于 ALS 和对照组，筛选了 247177 个潜在的 lncRNA-mRNA ceRNA 关系对。MALAT1调控的SYNRG、ITSN2、PICALM、AP3B1和AAK1基因可能在 ALS 的发病机制中起重要作用，这些结果可能有助于表征 ALS 的发病机制。